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Identification of Proteins that Regulate Radiation-induced Apoptosis in Murine Tumors with Wild Type p53

Authors
 Jinsil SEONG  ;  Hae Jin OH  ;  Wonwoo KIM  ;  Jeung Hee AN  ;  Jiyoung KIM 
Citation
 JOURNAL OF RADIATION RESEARCH, Vol.48(5) : 435-441, 2007 
Journal Title
 JOURNAL OF RADIATION RESEARCH 
ISSN
 0449-3060 
Issue Date
2007
MeSH
Animals ; Apoptosis/radiation effects* ; Apoptosis Regulatory Proteins/metabolism* ; Carcinoma, Hepatocellular/metabolism* ; Carcinoma, Hepatocellular/pathology ; Cell Line, Tumor ; Dose-Response Relationship, Radiation ; Female ; Liver Neoplasms/metabolism* ; Liver Neoplasms/pathology ; Mice ; Mice, Inbred C3H ; Ovarian Neoplasms/metabolism* ; Ovarian Neoplasms/pathology ; Radiation Dosage ; Tumor Suppressor Protein p53/metabolism*
Abstract
In this study, we investigated the molecular factors determining the induction of apoptosis by radiation. Two murine tumors syngeneic to C3H/HeJ mice were used: an ovarian carcinoma OCa-I, and a hepatocarcinoma HCa-I. Both have wild type p53, but display distinctly different radiosensitivity in terms of specific growth delay (12.7 d in OCa-I and 0.3 d in HCa-I) and tumor cure dose 50% (52.6 Gy in OCa-I and > 80 Gy in HCa-I). Eight-mm tumors on the thighs of mice were irradiated with 25 Gy and tumor samples were collected at regular time intervals after irradiation. The peak levels of apoptosis were 16.1 ± 0.6% in OCa-I and 0.2 ± 0.0% in HCa-I at 4 h after radiation, and this time point was used for subsequent proteomics analysis. Protein spots were identified by peptide mass fingerprinting with a focus on those related to apoptosis. In OCa-I tumors, radiation increased the expression of cytochrome c oxidase and Bcl2/adenovirus E1B-interacting 2 (Nip 2) protein higher than 3-fold. However in HCa-I, these two proteins showed no significant change. The results suggest that radiosensitivity in tumors with wild type p53 is regulated by a complex mechanism. Furthermore, these proteins could be molecular targets for a novel therapeutic strategy involving the regulation of radiosensitivity.
Files in This Item:
T200700268.pdf Download
DOI
10.1269/jrr.07015
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Seong, Jin Sil(성진실) ORCID logo https://orcid.org/0000-0003-1794-5951
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96520
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