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관절연골세포에서 인테그린과 SMAD 신호경로 사이의 신호교차반응 규명

Other Titles
 Cross-talk between Integrin and SMAD Signal Pathway in Articular Chondrocyte 
 한수봉  ;  김윤희  ;  박민성  ;  이진우 
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.10(2) : 65-75, 2007 
Journal Title
Journal of Korean Orthopaedic Research Society(대한정형외과연구학회지)
Issue Date
Articular chondrocyte ; Signal cross-talk ; Type II collagen ; TGF-β1 ; FAK ; SMAD 2/3
PURPOSE: Identifying the signal cross-talk between integrin signaling cascade and TGF-beta 1 signaling cascade in articular chondrocytes. MATERIALS AND METHODS: To analyze integrin or TGF-beta 1 mediated signaling pathways from extracellular stimuli, type II collagen was coated on the cell culture plate and TGF-beta 1 was added to cell culture media. Chondrocytes were cultured in the conditioned media with each or both stimuli. Altered activation of signaling proteins detected with western blot technique. RESULTS: More rapid attachment of cells was observed in the type II collagen coated group than non-coated group. The phosphorylated SMAD 2 and 3 were expressed in the type II collagen coated group and synergistically up-regulated phosphorylation in the co-treated group. The phosphorylated FAK at tyrosine 925 was activated by TGF-beta 1 treatment and synergistically up-regulated by both stimuli. But there was no meaningfully changed phosphorylation of extracellular signal regulated protein kinase (ERK) 1/2 and p38, as known downstream molecules of FAK cascade. CONCLUSION: This result means that SMAD 2, SMAD 3 and tyrosine 925 of FAK are involved in this signal cross-talking in articular chondrocytes.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Hahn, Soo Bong(한수봉)
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