Effect of Concentration and Exposure Duration of FBS on Parthenogenetic Development of Porcine Follicular Oocytes

Abstract

The aim of present experiment was to examine hatching rate as in vitro indicator of viability of porcine embryos before early stage embryo transfer such as zygotes or 2-cell stage embryos. Cumulus-oocyte complexes (COCs) collected from ovaries were matured in North Carolina State University 23 (NCSU-23) containing 10% porcine follicular fluid (pFF), 10 ng/ml epidermal growth factor (EGF), 10 ㎍/ml follicle stimulating hormone (FSH), 35 ㎍/ml luteinizing hormone (LH), and 1 mg/ml cysteine. After 24 hours, the COCs were transferred to the same medium without hormones.