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Diagnostic application of serum proteomic patterns in gastric cancer patients by ProteinChip surface-enhanced laser desorption/ionization time-of-flight mass spectrometry

Authors
 Lim JY  ;  Cho JY  ;  Kim HG  ;  Chang YS  ;  Paik YH 
Citation
 International Journal of Biological Markers, Vol.22(4) : 281-286, 2007 
Journal Title
 International Journal of Biological Markers 
ISSN
 0393-6155 
Issue Date
2007
Abstract
Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is one of thecurrently used techniques to identify biomarkers for cancers. This study was planned to establish a system to accurately distinguish gastric cancer patients by using SELDI-TOF-MS. A total of 100 serum samples obtained from 60 individuals with gastric cancer and 40 healthy individuals were screened. Protein expression profiles were expressed on CM10 ProteinChip arrays and analyzed. Peak intensities were analyzed with the Biomarker Wizard software to identify peaks showing significantly different intensities between normal and cancer groups. Classification analysis and construction of decision trees were done with the Biomarker Pattern software 5.0. Seventeen protein peaks showed significant differences between the two groups. The decision tree which gave the highest discrimination included four peaks at mass 5,919, 8,583, 10,286, and 13,758 as splitters. The sensitivity and specificity for classification of the decision tree were 96.7% (58/60) and 97.5% (39/40), respectively. When the protein biomarker pattern was tested on a blinded test set, it yielded a sensitivity of 93.3% (28/30) and a specificity of 90% (18/20). These results suggest that serum protein profiling by the SELDI system may distinguish gastric cancer patients from healthy controls with relatively high sensitivity and specificity.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
김호근(Kim, Ho Keun)
백용한(Paik, Yong Han)
임재윤(Lim, Jae Yun)
장윤수(Chang, Yoon Soo) ORCID logo https://orcid.org/0000-0003-3340-4223
조재용(Cho, Jae Yong) ORCID logo https://orcid.org/0000-0002-0926-1819
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96134
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