0 328

Cited 51 times in

Reduced imipenem susceptibility in Klebsiella pneumoniae clinical isolates with plasmid-mediated CMY-2 and DHA-1 β-lactamases co-mediated by porin loss

Authors
 Kyungwon Lee  ;  Dongeun Yong  ;  Yunsop Chong  ;  David M. Livermore  ;  Neil Woodford  ;  June Myung Kim  ;  Jong Hwa Yum  ;  Yeong Seon Choi 
Citation
 INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, Vol.29(2) : 201-206, 2007 
Journal Title
 INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS 
ISSN
 0924-8579 
Issue Date
2007
MeSH
Anti-Bacterial Agents/pharmacology* ; Bacterial Outer Membrane Proteins/analysis ; Bacterial Proteins/genetics ; Electrophoresis, Gel, Pulsed-Field ; Electrophoresis, Polyacrylamide Gel ; Humans ; Imipenem/pharmacology* ; Isoelectric Focusing ; Klebsiella pneumoniae/drug effects* ; Klebsiella pneumoniae/enzymology ; Klebsiella pneumoniae/genetics ; Microbial Sensitivity Tests ; Plasmids* ; Porins/analysis ; Porins/physiology* ; beta-Lactamases/genetics*
Abstract
We investigated the resistance mechanisms and clonality among 42 imipenem-non-susceptible Klebsiella pneumoniae isolated at a tertiary care hospital in Korea. Two isolates had blaVIM-2 alleles, whereas blaCMY-2- and blaDHA-1-like alleles were detected in 24 and 16 isolates, respectively, with these enzymes confirmed by sequencing for representative isolates. Transfer of blaCMY-2 and blaDHA-1 was achieved by conjugation. Addition of 300 mg/L 3-aminophenylboronic acid (APB) reduced the minimum inhibitory concentration for 90% of the organisms (MIC90) of imipenem and meropenem eight- and four-fold, respectively, for the blaCMY-2- and blaDHA-1-positive isolates, confirming the role of these enzymes in resistance. SDS-PAGE of outer membrane proteins for representative isolates showed lack or greatly diminished expression of OmpK35 and OmpK36 porins. Pulsed-field gel electrophoresis of XbaI-restricted genomic DNA revealed two closely related clusters among 23 blaCMY-2-positive isolates, whereas those with blaDHA-1 were more heterogeneous. In conclusion, reduced imipenem susceptibility among K. pneumoniae at this Korean hospital was largely co-mediated by production of plasmid-mediated AmpC β-lactamases along with lack or greatly diminished expression of OmpK35 and OmpK36 porins.
Full Text
http://www.sciencedirect.com/science/article/pii/S0924857906004109
DOI
10.1016/j.ijantimicag.2006.09.006
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Research Institute of Bacterial Resistance (세균내성연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, June Myung(김준명)
Yum, Jong Hwa(염종화)
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96128
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse