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Reduced imipenem susceptibility in Klebsiella pneumoniae clinical isolates with plasmid-mediated CMY-2 and DHA-1 β-lactamases co-mediated by porin loss

DC FieldValueLanguage
dc.contributor.author김준명-
dc.contributor.author염종화-
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.contributor.author정윤섭-
dc.date.accessioned2014-12-21T16:40:16Z-
dc.date.available2014-12-21T16:40:16Z-
dc.date.issued2007-
dc.identifier.issn0924-8579-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/96128-
dc.description.abstractWe investigated the resistance mechanisms and clonality among 42 imipenem-non-susceptible Klebsiella pneumoniae isolated at a tertiary care hospital in Korea. Two isolates had blaVIM-2 alleles, whereas blaCMY-2- and blaDHA-1-like alleles were detected in 24 and 16 isolates, respectively, with these enzymes confirmed by sequencing for representative isolates. Transfer of blaCMY-2 and blaDHA-1 was achieved by conjugation. Addition of 300 mg/L 3-aminophenylboronic acid (APB) reduced the minimum inhibitory concentration for 90% of the organisms (MIC90) of imipenem and meropenem eight- and four-fold, respectively, for the blaCMY-2- and blaDHA-1-positive isolates, confirming the role of these enzymes in resistance. SDS-PAGE of outer membrane proteins for representative isolates showed lack or greatly diminished expression of OmpK35 and OmpK36 porins. Pulsed-field gel electrophoresis of XbaI-restricted genomic DNA revealed two closely related clusters among 23 blaCMY-2-positive isolates, whereas those with blaDHA-1 were more heterogeneous. In conclusion, reduced imipenem susceptibility among K. pneumoniae at this Korean hospital was largely co-mediated by production of plasmid-mediated AmpC β-lactamases along with lack or greatly diminished expression of OmpK35 and OmpK36 porins.-
dc.description.statementOfResponsibilityopen-
dc.format.extent201~206-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnti-Bacterial Agents/pharmacology*-
dc.subject.MESHBacterial Outer Membrane Proteins/analysis-
dc.subject.MESHBacterial Proteins/genetics-
dc.subject.MESHElectrophoresis, Gel, Pulsed-Field-
dc.subject.MESHElectrophoresis, Polyacrylamide Gel-
dc.subject.MESHHumans-
dc.subject.MESHImipenem/pharmacology*-
dc.subject.MESHIsoelectric Focusing-
dc.subject.MESHKlebsiella pneumoniae/drug effects*-
dc.subject.MESHKlebsiella pneumoniae/enzymology-
dc.subject.MESHKlebsiella pneumoniae/genetics-
dc.subject.MESHMicrobial Sensitivity Tests-
dc.subject.MESHPlasmids*-
dc.subject.MESHPorins/analysis-
dc.subject.MESHPorins/physiology*-
dc.subject.MESHbeta-Lactamases/genetics*-
dc.titleReduced imipenem susceptibility in Klebsiella pneumoniae clinical isolates with plasmid-mediated CMY-2 and DHA-1 β-lactamases co-mediated by porin loss-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorKyungwon Lee-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorYunsop Chong-
dc.contributor.googleauthorDavid M. Livermore-
dc.contributor.googleauthorNeil Woodford-
dc.contributor.googleauthorJune Myung Kim-
dc.contributor.googleauthorJong Hwa Yum-
dc.contributor.googleauthorYeong Seon Choi-
dc.identifier.doi10.1016/j.ijantimicag.2006.09.006-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00953-
dc.contributor.localIdA02352-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.contributor.localIdA03679-
dc.relation.journalcodeJ01088-
dc.identifier.eissn1872-7913-
dc.identifier.pmid17204406-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0924857906004109-
dc.contributor.alternativeNameKim, June Myung-
dc.contributor.alternativeNameYum, Jong Hwa-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameChong, Yun Sop-
dc.contributor.affiliatedAuthorKim, June Myung-
dc.contributor.affiliatedAuthorYum, Jong Hwa-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorChong, Yun Sop-
dc.rights.accessRightsnot free-
dc.citation.volume29-
dc.citation.number2-
dc.citation.startPage201-
dc.citation.endPage206-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, Vol.29(2) : 201-206, 2007-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Research Institute of Bacterial Resistance (세균내성연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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