Cited 55 times in
E1A- and E1B-double mutant replicating adenovirus elicits enhanced oncolytic and antitumor effects
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김재성 | - |
dc.contributor.author | 김주항 | - |
dc.contributor.author | 김평환 | - |
dc.contributor.author | 윤채옥 | - |
dc.contributor.author | 최경주 | - |
dc.date.accessioned | 2014-12-21T16:39:16Z | - |
dc.date.available | 2014-12-21T16:39:16Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1043-0342 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96096 | - |
dc.description.abstract | Gene-modified replication-competent adenoviruses (Ads) are emerging as a promising new modality for the treatment of cancer. We have previously shown that E1B 19kDa and E1B 55kDa gene-deleted Ad (Ad-ΔE1B19/55) exhibits improved tumor-specific replication and cell lysis, leading to an enhanced antitumor effect. In an effort to increase cancer cell selectivity of a replicating adenovirus, we first generated 11 E1A mutant Ads (Ad-E1mt1 to Ad-E1mt11) with deletion or substitution in retinoblastoma (pRb)-binding sites of E1A. Of these, Ad-E1mt7 demonstrated significant improvement in cytopathic effect (CPE) and viral replication in a cancer cell-specific manner. To further enhance the cancer cell specificity of Ad-E1mt7, Ad-ΔE1Bmt7 was generated, in which both the E1B 19kDa and E1B 55kDa genes were deleted. As assessed in CPE assay and immunoblot analysis for Ad fiber expression, Ad-ΔE1Bmt7 exerted marked enhancement in cancer cell-specific killing as well as viral replication in comparison with its comparative controls (Ad-E1mt7, Ad-ΔE1B55). Furthermore, the growth of established human cervical carcinoma in nude mice was significantly suppressed by intratumoral injection of Ad-ΔE1Bmt7. In summary, we have developed an oncolytic adenovirus with a significantly improved therapeutic profile for cancer treatment. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 773~786 | - |
dc.relation.isPartOf | HUMAN GENE THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae*/genetics | - |
dc.subject.MESH | Adenoviridae*/physiology | - |
dc.subject.MESH | Adenovirus E1A Proteins/genetics* | - |
dc.subject.MESH | Adenovirus E1B Proteins/genetics* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Gene Deletion | - |
dc.subject.MESH | Genetic Therapy | - |
dc.subject.MESH | Genetic Vectors* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Kidney/cytology | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Nude | - |
dc.subject.MESH | Mutation | - |
dc.subject.MESH | Neoplasm Transplantation | - |
dc.subject.MESH | Neoplasms, Experimental/pathology | - |
dc.subject.MESH | Neoplasms, Experimental/therapy | - |
dc.subject.MESH | Oncolytic Virotherapy* | - |
dc.subject.MESH | Virus Replication* | - |
dc.title | E1A- and E1B-double mutant replicating adenovirus elicits enhanced oncolytic and antitumor effects | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Medical Education (의학교육학) | - |
dc.contributor.googleauthor | Jaesung Kim | - |
dc.contributor.googleauthor | Joo-Hang Kim | - |
dc.contributor.googleauthor | Chae-Ok Yun | - |
dc.contributor.googleauthor | Pyung-Hwan Kim | - |
dc.contributor.googleauthor | Kyung-Ju Choi | - |
dc.identifier.doi | 10.1089/hum.2006.167 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00945 | - |
dc.contributor.localId | A01088 | - |
dc.contributor.localId | A02614 | - |
dc.contributor.localId | A04034 | - |
dc.contributor.localId | A00860 | - |
dc.relation.journalcode | J01006 | - |
dc.identifier.eissn | 1557-7422 | - |
dc.identifier.pmid | 17725410 | - |
dc.identifier.url | http://online.liebertpub.com/doi/abs/10.1089/hum.2006.167 | - |
dc.contributor.alternativeName | Kim, Jae Sung | - |
dc.contributor.alternativeName | Kim, Joo Hang | - |
dc.contributor.alternativeName | Kim, Pyung Hwan | - |
dc.contributor.alternativeName | Yun, Chae Ok | - |
dc.contributor.alternativeName | Choi, Kyung Ju | - |
dc.contributor.affiliatedAuthor | Kim, Joo Hang | - |
dc.contributor.affiliatedAuthor | Kim, Pyung Hwan | - |
dc.contributor.affiliatedAuthor | Yun, Chae Ok | - |
dc.contributor.affiliatedAuthor | Choi, Kyung Ju | - |
dc.contributor.affiliatedAuthor | Kim, Jaesung | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 18 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 773 | - |
dc.citation.endPage | 786 | - |
dc.identifier.bibliographicCitation | HUMAN GENE THERAPY, Vol.18(9) : 773-786, 2007 | - |
dc.identifier.rimsid | 35430 | - |
dc.type.rims | ART | - |
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