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E1A- and E1B-double mutant replicating adenovirus elicits enhanced oncolytic and antitumor effects

DC Field Value Language
dc.contributor.author김재성-
dc.contributor.author김주항-
dc.contributor.author김평환-
dc.contributor.author윤채옥-
dc.contributor.author최경주-
dc.date.accessioned2014-12-21T16:39:16Z-
dc.date.available2014-12-21T16:39:16Z-
dc.date.issued2007-
dc.identifier.issn1043-0342-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/96096-
dc.description.abstractGene-modified replication-competent adenoviruses (Ads) are emerging as a promising new modality for the treatment of cancer. We have previously shown that E1B 19kDa and E1B 55kDa gene-deleted Ad (Ad-ΔE1B19/55) exhibits improved tumor-specific replication and cell lysis, leading to an enhanced antitumor effect. In an effort to increase cancer cell selectivity of a replicating adenovirus, we first generated 11 E1A mutant Ads (Ad-E1mt1 to Ad-E1mt11) with deletion or substitution in retinoblastoma (pRb)-binding sites of E1A. Of these, Ad-E1mt7 demonstrated significant improvement in cytopathic effect (CPE) and viral replication in a cancer cell-specific manner. To further enhance the cancer cell specificity of Ad-E1mt7, Ad-ΔE1Bmt7 was generated, in which both the E1B 19kDa and E1B 55kDa genes were deleted. As assessed in CPE assay and immunoblot analysis for Ad fiber expression, Ad-ΔE1Bmt7 exerted marked enhancement in cancer cell-specific killing as well as viral replication in comparison with its comparative controls (Ad-E1mt7, Ad-ΔE1B55). Furthermore, the growth of established human cervical carcinoma in nude mice was significantly suppressed by intratumoral injection of Ad-ΔE1Bmt7. In summary, we have developed an oncolytic adenovirus with a significantly improved therapeutic profile for cancer treatment.-
dc.description.statementOfResponsibilityopen-
dc.format.extent773~786-
dc.relation.isPartOfHUMAN GENE THERAPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenoviridae*/genetics-
dc.subject.MESHAdenoviridae*/physiology-
dc.subject.MESHAdenovirus E1A Proteins/genetics*-
dc.subject.MESHAdenovirus E1B Proteins/genetics*-
dc.subject.MESHAnimals-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Line-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHGene Deletion-
dc.subject.MESHGenetic Therapy-
dc.subject.MESHGenetic Vectors*-
dc.subject.MESHHumans-
dc.subject.MESHKidney/cytology-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Nude-
dc.subject.MESHMutation-
dc.subject.MESHNeoplasm Transplantation-
dc.subject.MESHNeoplasms, Experimental/pathology-
dc.subject.MESHNeoplasms, Experimental/therapy-
dc.subject.MESHOncolytic Virotherapy*-
dc.subject.MESHVirus Replication*-
dc.titleE1A- and E1B-double mutant replicating adenovirus elicits enhanced oncolytic and antitumor effects-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Education (의학교육학)-
dc.contributor.googleauthorJaesung Kim-
dc.contributor.googleauthorJoo-Hang Kim-
dc.contributor.googleauthorChae-Ok Yun-
dc.contributor.googleauthorPyung-Hwan Kim-
dc.contributor.googleauthorKyung-Ju Choi-
dc.identifier.doi10.1089/hum.2006.167-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00945-
dc.contributor.localIdA01088-
dc.contributor.localIdA02614-
dc.contributor.localIdA04034-
dc.contributor.localIdA00860-
dc.relation.journalcodeJ01006-
dc.identifier.eissn1557-7422-
dc.identifier.pmid17725410-
dc.identifier.urlhttp://online.liebertpub.com/doi/abs/10.1089/hum.2006.167-
dc.contributor.alternativeNameKim, Jae Sung-
dc.contributor.alternativeNameKim, Joo Hang-
dc.contributor.alternativeNameKim, Pyung Hwan-
dc.contributor.alternativeNameYun, Chae Ok-
dc.contributor.alternativeNameChoi, Kyung Ju-
dc.contributor.affiliatedAuthorKim, Joo Hang-
dc.contributor.affiliatedAuthorKim, Pyung Hwan-
dc.contributor.affiliatedAuthorYun, Chae Ok-
dc.contributor.affiliatedAuthorChoi, Kyung Ju-
dc.contributor.affiliatedAuthorKim, Jaesung-
dc.rights.accessRightsnot free-
dc.citation.volume18-
dc.citation.number9-
dc.citation.startPage773-
dc.citation.endPage786-
dc.identifier.bibliographicCitationHUMAN GENE THERAPY, Vol.18(9) : 773-786, 2007-
dc.identifier.rimsid35430-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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