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Rat homolog of PinX1 is a nucleolar protein involved in the regulation of telomere length

 Bong-Kyeong Oh  ;  So-Mi Yoon  ;  Young Nyun Park  ;  Chan-Hee Lee 
 GENE, Vol.400(1-2) : 35-43, 2007 
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Animals ; Cell Nucleolus/metabolism ; Cells, Cultured ; Cellular Senescence ; Humans ; Mice ; Molecular Sequence Data ; NIH 3T3 Cells ; Nuclear Proteins/genetics* ; Nuclear Proteins/metabolism ; Rats ; Sequence Homology, Amino Acid ; Telomere/ultrastructure* ; Transfection ; Tumor Suppressor Proteins/genetics* ; Tumor Suppressor Proteins/metabolism
Human PinX1 involves in regulation of telomere length. Here, we describe the function of a rat homolog of PinX1. Rat PinX1 (rPinX1) was cloned from WB-F344, a rat hepatic stem-like epithelial cell. It encodes a protein of 331 amino acids with 70% homology to human PinX1 and 91% homology to mouse. Northern analysis revealed that rPinX1 is expressed in both somatic and germ tissues, most abundantly in heart, liver and testis. Co-localization with a nucleolar protein, fibrillarin, showed that rPinX1 resides in the nucleolus. Analysis of truncated mutants revealed that an internal K,E/D region seems to be important for nucleolar localization. A stable cell line expressing rPinX1 was established in NIH3T3, a mouse-transformed embryonic fibroblast cell line, and stable cells were subcultured for more than 150 population doublings. The growth of stable rPinX1 cells slowed down at late passages, and a fraction of these cells exhibited increased size and stained positively for senescence-associated β-galactosidase. Overexpression of rPinX1 in NIH3T3 cells resulted in gradual telomere shortening over successive passages. However, the telomeric 3′ overhang was not altered by PinX1 expression. This study demonstrates that a rat homolog of human PinX1 is a nucleolar protein, and that overexpression of rPinX1 induces cellular senescence and telomere shortening, but has no effect on 3′ overhang length. The function of PinX1 in regulating telomere length is conserved in rodents, and this study may provide insight into the mechanism by which a nucleolar protein can regulate telomere length.
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1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
Oh, Bong Kyeong(오봉경)
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