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Two‐pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respiration

Authors
 Sang Sun Yoon  ;  Ahmet C Karabulut  ;  Daniel J Hassett  ;  Herbert P Schweizer  ;  Kyoung‐Hee Choi  ;  Benjamin Gaston  ;  Michael J Schurr  ;  Patricia J Kiley  ;  Michael L Howell  ;  Andrew B Herr  ;  Stephanie L Groce  ;  Sergei V Lymar  ;  Robert F Hennigan  ;  John D Lipscomb 
Citation
 EMBO JOURNAL, Vol.26(15) : 3662-3672, 2007 
Journal Title
EMBO JOURNAL
ISSN
 0261-4189 
Issue Date
2007
MeSH
Anaerobiosis ; Bacterial Proteins/genetics ; Bacterial Proteins/physiology ; Cystic Fibrosis/microbiology* ; Electrophoresis, Gel, Two-Dimensional ; Mutation ; Nitric Oxide/metabolism* ; Pseudomonas aeruginosa/growth & development ; Pseudomonas aeruginosa/metabolism ; Pseudomonas aeruginosa/physiology* ; Spectrum Analysis/methods ; Transcription, Genetic
Abstract
Protection from NO gas, a toxic byproduct of anaerobic respiration in Pseudomonas aeruginosa, is mediated by nitric oxide (NO) reductase (NOR), the norCB gene product. Nevertheless, a norCB mutant that accumulated ∼13.6 μM NO paradoxically survived anaerobic growth. Transcription of genes encoding nitrate and nitrite reductases, the enzymes responsible for NO production, was reduced >50‐ and 2.5‐fold in the norCB mutant. This was due, in part, to a predicted compromise of the [4Fe–4S]2+ cluster in the anaerobic regulator ANR by physiological NO levels, resulting in an inability to bind to its cognate promoter DNA sequences. Remarkably, two O2‐dependent dioxygenases, homogentisate‐1,2‐dioxygenase (HmgA) and 4‐hydroxyphenylpyruvate dioxygenase (Hpd), were derepressed in the norCB mutant. Electron paramagnetic resonance studies showed that HmgA and Hpd bound NO avidly, and helped protect the norCB mutant in anaerobic biofilms. These data suggest that protection of a P. aeruginosa norCB mutant against anaerobic NO toxicity occurs by both control of NO supply and reassignment of metabolic enzymes to the task of NO sequestration.
Files in This Item:
T200705146.pdf Download
DOI
10.1038/sj.emboj.7601787
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95963
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