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Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

Authors
 Il Kwon Bae  ;  You-Nae Lee  ;  Hasik Youn  ;  Hyoung Jin Kim  ;  Sang Hee Lee  ;  Jung Hun Lee  ;  Seong Geun Hong  ;  Seok Hoon Jeong 
Citation
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.58(4) : 465-468, 2007 
Journal Title
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 
ISSN
 0732-8893 
Issue Date
2007
MeSH
Cloning, Molecular ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Female ; Humans ; Integrons ; Kinetics ; Klebsiella Infections/microbiology ; Klebsiella pneumoniae/drug effects* ; Klebsiella pneumoniae/genetics* ; Klebsiella pneumoniae/isolation & purification ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Sequence Analysis, DNA ; Sputum/microbiology ; beta-Lactam Resistance/genetics* ; beta-Lactamases/chemistry ; beta-Lactamases/genetics* ; beta-Lactamases/isolation & purification ; beta-Lactamases/metabolism ; beta-Lactams/pharmacology
Abstract
The present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 β-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5–aac(6′)-IIa–blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most β-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4.
Full Text
http://www.sciencedirect.com/science/article/pii/S0732889307001034
DOI
10.1016/j.diagmicrobio.2007.02.013
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Bae, Il Kwon(배일권)
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95951
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