Cited 49 times in
Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae
DC Field | Value | Language |
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dc.contributor.author | 배일권 | - |
dc.contributor.author | 정석훈 | - |
dc.date.accessioned | 2014-12-21T16:34:44Z | - |
dc.date.available | 2014-12-21T16:34:44Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0732-8893 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95951 | - |
dc.description.abstract | The present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 β-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5–aac(6′)-IIa–blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most β-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 465~468 | - |
dc.relation.isPartOf | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cloning, Molecular | - |
dc.subject.MESH | DNA, Bacterial/chemistry | - |
dc.subject.MESH | DNA, Bacterial/genetics | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Integrons | - |
dc.subject.MESH | Kinetics | - |
dc.subject.MESH | Klebsiella Infections/microbiology | - |
dc.subject.MESH | Klebsiella pneumoniae/drug effects* | - |
dc.subject.MESH | Klebsiella pneumoniae/genetics* | - |
dc.subject.MESH | Klebsiella pneumoniae/isolation & purification | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Sequence Analysis, DNA | - |
dc.subject.MESH | Sputum/microbiology | - |
dc.subject.MESH | beta-Lactam Resistance/genetics* | - |
dc.subject.MESH | beta-Lactamases/chemistry | - |
dc.subject.MESH | beta-Lactamases/genetics* | - |
dc.subject.MESH | beta-Lactamases/isolation & purification | - |
dc.subject.MESH | beta-Lactamases/metabolism | - |
dc.subject.MESH | beta-Lactams/pharmacology | - |
dc.title | Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Il Kwon Bae | - |
dc.contributor.googleauthor | You-Nae Lee | - |
dc.contributor.googleauthor | Hasik Youn | - |
dc.contributor.googleauthor | Hyoung Jin Kim | - |
dc.contributor.googleauthor | Sang Hee Lee | - |
dc.contributor.googleauthor | Jung Hun Lee | - |
dc.contributor.googleauthor | Seong Geun Hong | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.identifier.doi | 10.1016/j.diagmicrobio.2007.02.013 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01802 | - |
dc.contributor.localId | A03619 | - |
dc.relation.journalcode | J00731 | - |
dc.identifier.eissn | 1879-0070 | - |
dc.identifier.pmid | 17467221 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0732889307001034 | - |
dc.contributor.alternativeName | Bae, Il Kwon | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Bae, Il Kwon | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 58 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 465 | - |
dc.citation.endPage | 468 | - |
dc.identifier.bibliographicCitation | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.58(4) : 465-468, 2007 | - |
dc.identifier.rimsid | 35326 | - |
dc.type.rims | ART | - |
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