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Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

DC FieldValueLanguage
dc.contributor.author배일권-
dc.contributor.author정석훈-
dc.date.accessioned2014-12-21T16:34:44Z-
dc.date.available2014-12-21T16:34:44Z-
dc.date.issued2007-
dc.identifier.issn0732-8893-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/95951-
dc.description.abstractThe present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 β-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5–aac(6′)-IIa–blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most β-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4.-
dc.description.statementOfResponsibilityopen-
dc.format.extent465~468-
dc.relation.isPartOfDIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHDNA, Bacterial/chemistry-
dc.subject.MESHDNA, Bacterial/genetics-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHIntegrons-
dc.subject.MESHKinetics-
dc.subject.MESHKlebsiella Infections/microbiology-
dc.subject.MESHKlebsiella pneumoniae/drug effects*-
dc.subject.MESHKlebsiella pneumoniae/genetics*-
dc.subject.MESHKlebsiella pneumoniae/isolation & purification-
dc.subject.MESHMicrobial Sensitivity Tests-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHSequence Analysis, DNA-
dc.subject.MESHSputum/microbiology-
dc.subject.MESHbeta-Lactam Resistance/genetics*-
dc.subject.MESHbeta-Lactamases/chemistry-
dc.subject.MESHbeta-Lactamases/genetics*-
dc.subject.MESHbeta-Lactamases/isolation & purification-
dc.subject.MESHbeta-Lactamases/metabolism-
dc.subject.MESHbeta-Lactams/pharmacology-
dc.titleGenetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorIl Kwon Bae-
dc.contributor.googleauthorYou-Nae Lee-
dc.contributor.googleauthorHasik Youn-
dc.contributor.googleauthorHyoung Jin Kim-
dc.contributor.googleauthorSang Hee Lee-
dc.contributor.googleauthorJung Hun Lee-
dc.contributor.googleauthorSeong Geun Hong-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.identifier.doi10.1016/j.diagmicrobio.2007.02.013-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01802-
dc.contributor.localIdA03619-
dc.relation.journalcodeJ00731-
dc.identifier.eissn1879-0070-
dc.identifier.pmid17467221-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0732889307001034-
dc.contributor.alternativeNameBae, Il Kwon-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.affiliatedAuthorBae, Il Kwon-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.rights.accessRightsnot free-
dc.citation.volume58-
dc.citation.number4-
dc.citation.startPage465-
dc.citation.endPage468-
dc.identifier.bibliographicCitationDIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.58(4) : 465-468, 2007-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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