1 149

Cited 36 times in

Investigation on biodegradable PLGA scaffold with various pore size structure for skin tissue engineering

Authors
 Jun Jae Lee  ;  Sang-Gil Lee  ;  Jeong Koo Kim  ;  Young Il Yang  ;  Jong Chul Park 
Citation
 CURRENT APPLIED PHYSICS, Vol.7(s1) : 37-40, 2007 
Journal Title
 CURRENT APPLIED PHYSICS 
ISSN
 1567-1739 
Issue Date
2007
Abstract
For tissue regeneration, highly open porous polymer matrices are required for high-density cell seeding, as well as sufficient nutrient and oxygen supply to the cells in the 3-D matrices. In this study, three types of scaffolds containing three different pore sizes (uniform-pore size, 2-layer pore size and multi-pore size) were prepared. We used human dermal fibroblast cells to investigate cell attachment and proliferation with the prepared specimens. Not only DNA quantity measurement of the cells but also the number of cells within the cross-sectional area of the scaffold was investigated. In the DNA quantity test, the multi-pore size scaffold contained a 1.77 times larger amount than the uniform-pore size scaffold for 14 days culture. For the cell-counting assessment, the multi-pore size scaffold contained about 2.24 times more cells than the uniform-pore size scaffold in the middle sectioned area for 14 days. To prevent the generation of a polymer skin layer on the surface of the scaffolds, PLGA scaffolds were fabricated by a two-step molding method. No skin layer was observed in the scaffold. Various pore size specimens tended to degrade more and faster than uniform-pore size specimens in PBS solution. Chemical treatment on the surface of the specimen enhances cytocompatibility of the scaffold.
Full Text
http://www.sciencedirect.com/science/article/pii/S1567173906002203
DOI
10.1016/j.cap.2006.11.011
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95927
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse