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Regulation of German cockroach extract-induced IL-8 expression in human airway epithelial cells

 K. E. Lee  ;  J. W. Kim  ;  M. H. Sohn  ;  T. S. Yong  ;  K. E. Kim  ;  K. Y. Jeong 
 Clinical and Experimental Allergy, Vol.37(9) : 1364-1373, 2007 
Journal Title
 Clinical and Experimental Allergy 
Issue Date
BACKGROUND: Cockroaches have been known as a cause of respiratory allergies such as asthma. IL-8 plays an integral role in the coordination and persistence of the inflammatory process in the chronic inflammation of the airways in asthma. OBJECTIVE: We investigated the mechanism by which German cockroach extract (GCE) triggers IL-8 release from human airway epithelial cells. METHODS: Chemical inhibitors were pretreated before addition of GCE for promoter activity and protein synthesis of IL-8. The Transcriptional activity of IL-8 promoter was analysed by mutational, deletional anaylsis and electrophoretic mobility shift assay (EMSA). RESULTS: Stimulation of H292 cells with GCE resulted in a time- and concentration-dependent induction of IL-8 transcription and protein synthesis. IL-8 promoter deletion analysis indicated that position -132 to +41 was essential for GCE-induced IL-8 transcription, and mutants with substitutions in activator protein (AP)-1, nuclear factor (NF)-IL6 and NF-kappaB-binding sites revealed a requirement for NF-kappaB and NF-IL6, but not AP-1, in GCE-induced activation of the IL-8 promoter. The DNA-binding activities of NF-kappaB and NF-IL6 were induced by GCE, as determined by EMSA. The chemical inhibition of extracellular signal-regulated kinase (ERK) attenuated GCE-induced transcriptional activity and protein synthesis. In addition, through aprotinin treatment and PAR2 small interfering RNA transfection, it was proven that protease of GCE is consistent with the regulation of GCE-induced IL-8. CONCLUSION: We conclude that GCE with protease activity-induced IL-8 expression is regulated by transcriptional activation of NF-kappaB and NF-IL6 coordinating with the ERK pathway in human airway epithelial cells.
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5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
김규언(Kim, Kyu Earn)
김재우(Kim, Jae Woo) ORCID logo https://orcid.org/0000-0001-5456-9495
손명현(Sohn, Myung Hyun) ORCID logo https://orcid.org/0000-0002-2478-487X
용태순(Yong, Tai Soon) ORCID logo https://orcid.org/0000-0002-3445-0769
정경용(Jeong, Kyoung Yong) ORCID logo https://orcid.org/0000-0001-9887-1426
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