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Determination of adipose-derived stem cell application on photo-aged fibroblasts, based on paracrine function

 Seung Yong Song  ;  Ji Eun Jung  ;  Yeo Reum Jeon  ;  Kwan Chul Tark  ;  Dae Hyun Lew 
 CYTOTHERAPY, Vol.13(3) : 378-384, 2011 
Journal Title
Issue Date
Adipose Tissue/cytology* ; Adolescent ; Annexin A5/metabolism ; Apoptosis/radiation effects ; Cell Proliferation/radiation effects ; Cell Shape/radiation effects ; Cellular Senescence/radiation effects* ; Child ; Collagen Type I/genetics ; Collagen Type I/metabolism ; Cyclin-Dependent Kinase Inhibitor p16/metabolism ; Female ; Fibroblasts/cytology* ; Fibroblasts/metabolism ; Fibroblasts/radiation effects* ; Flow Cytometry ; Gene Expression Regulation/radiation effects ; Humans ; Male ; Matrix Metalloproteinase 1/genetics ; Matrix Metalloproteinase 1/metabolism ; Paracrine Communication/radiation effects* ; Reproducibility of Results ; Stem Cells/cytology* ; Stem Cells/metabolism ; Ultraviolet Rays* ; Young Adult ; beta-Galactosidase/metabolism
adipose-derived stem cells ; anti-aging ; apoptosis ; photo-aged fibroblast
BACKGROUND AIMS: Adipose-derived stem cells (ASC) are known to be able to restore injured tissue via differentiation and paracrine effects. In this study, we investigated the effect of ASC on photo-aged human dermal fibroblasts (HDF) based on paracrine function. In particular, we wanted to determine a more effective method of ASC application and the fate of the photo-aged fibroblasts. METHODS: We compared two application methods of ASC: transwell and conditioned medium culture with photo-aged fibroblasts. Proliferation rate, collagen synthesis, matrix metalloproteinase (MMP) production and expression of p16 were measured by real-time polymerase chain reaction (PCR) after culture. Flow cytometry for apoptosis assay was also conducted to determine the fate of the photo-aged fibroblasts. RESULTS: ASC induced proliferation of photo-aged HDF and type I collagen production and decreased MMP-1 production and expression of p16. In an apoptosis assay, ASC converted necrotic or late apoptotic cells to early apoptotic cells. These results were similar in both experimental groups. CONCLUSIONS: The results indicate that the paracrine effects of ASC may have a role that is as important as cell-to-cell communication between ASC and fibroblasts. We believe that conditioned medium may be a useful material for anti-aging skin therapy instead of cell therapy. Also, ASC might have an anti-aging effect on photo-aged fibroblasts even at a genetic level.
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1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Song, Seung Yong(송승용) ORCID logo https://orcid.org/0000-0002-3145-7463
Lew, Dae Hyun(유대현)
Tark, Kwan Chul(탁관철)
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