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Cross-species hybridization of microarrays for studying tumor transcriptome of brain metastasis

Authors
 Eun Sung Park  ;  Sun Jin Kim  ;  Seung Wook Kim  ;  Se-Lyun Yoon  ;  Sun-Hee Leem  ;  Sang-Bae Kim  ;  Soo Mi Kim Yun-Yong Parka, Jae-Ho Cheonga, Hyun Goo Wooe, Gordon B. Millsa,1, Isaiah J. Fidlerb,2, and Ju-Seog Leea, 
Citation
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.108(42) : 17456-17461, 2011 
Journal Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN
 0027-8424 
Issue Date
2011
MeSH
Animals ; Astrocytes/metabolism ; Base Sequence ; Brain Neoplasms/genetics* ; Brain Neoplasms/metabolism ; Brain Neoplasms/secondary* ; Cell Line, Tumor ; Coculture Techniques ; DNA Methylation ; DNA Primers/genetics ; DNA, Neoplasm/genetics ; Epigenesis, Genetic ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Neurons/metabolism ; Nucleic Acid Hybridization ; Oligonucleotide Array Sequence Analysis ; Species Specificity ; Transcriptome ; Transplantation, Heterologous ; Tumor Microenvironment/genetics
Keywords
genomics ; tumor microenvironment ; stromal cells ; DNA methylation
Abstract
Although the importance of the cellular microenvironment (soil) during invasion and metastasis of cancer cells (seed) has been well-recognized, technical challenges have limited the ability to assess the influence of the microenvironment on cancer cells at the molecular level. Here, we show that an experimental strategy, competitive cross-species hybridization of microarray experiments, can characterize the influence of different microenvironments on cancer cells by independently extracting gene expression data of cancer and host cells when human cancer cells were xenografted into different organ sites of immunocompromised mice. Surprisingly, the analysis of gene expression data showed that the brain microenvironment induces complete reprogramming of metastasized cancer cells, resulting in a gain of neuronal cell characteristics and mimicking neurogenesis during development. We also show that epigenetic changes coincide with transcriptional reprogramming in cancer cells. These observations provide proof of principle for competitive cross-species hybridization of microarray experiments to characterize the effect of the microenvironment on tumor cell behavior.
Files in This Item:
T201104717.pdf Download
DOI
10.1073/pnas.1114210108
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Eun Sung(박은성)
Cheong, Jae Ho(정재호) ORCID logo https://orcid.org/0000-0002-1703-1781
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94787
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