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Gallic acid, a histone acetyltransferase inhibitor, suppresses β-amyloid neurotoxicity by inhibiting microglial-mediated neuroinflammation

 Mi-Jeong Kim  ;  Ah-Reum Seong  ;  Ho-Geun Yoon  ;  Woo Jin Jun  ;  Jeongmin Lee  ;  Young Jun Kim  ;  Yoo-Hyun Lee  ;  Cheng-Hao Jin  ;  Jung-Yoon Yoo 
 Molecular Nutrition & Food Research, Vol.55(12) : 1798-1808, 2011 
Journal Title
 Molecular Nutrition & Food Research 
Issue Date
SCOPE: We examined the biological effect of gallic acid (GA) as a nuclear factor (NF)-κB acetyltransferase inhibitor on microglial-mediated β-amyloid neurotoxicity and restorative effects on the Aβ-induced cognitive dysfunction. METHODS AND RESULTS: The protective effects of GA on the survival of neuronal cells were assessed with an MTT assay and a co-culture system. For the co-culture experiments, both BV-2 and primary microglia cells were treated with GA prior to Aβ stimulation, and conditioned media were transferred to Neuro-2A cells. The mRNA and protein levels of inflammatory cytokines in both microglia and Neuro-2A cells were assessed with real-time polymerase chain reaction and western blotting. Inhibition of nuclear factor kappa B (NF-κB) acetylation with GA treatment resulted in reduced cytokine production in microglia cells and protection of neuronal cells from Aβ-induced neurotoxicity. Furthermore, we observed a restorative effect of GA on Aβ-induced cognitive dysfunction in mice with Y-maze and passive avoidance tests. Finally, we found that GA treatment efficiently blocked neuronal cell death by downregulating the expression of cytokines and the in vivo levels of NF-κB acetylation. CONCLUSION: These results suggest that selective inhibition of NF-κB acetylation by the histone acetyltransferase inhibitor GA is a possible therapeutic approach for alleviating the inflammatory progression of Alzheimer disease
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1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Biochemistry & Molecular Biology (생화학-분자생물학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Life Science (의생명과학부)
Yonsei Authors
유정윤(Yoo, Jung Yoon) ; 윤호근(Yoon, Ho Geun)
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