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Dissemination of IMP-6 metallo-β-lactamase-producing Pseudomonas aeruginosa sequence type 235 in Korea.

Authors
 Yoonmi Seok  ;  Il Kwon Bae  ;  Seok Hoon Jeong  ;  Soo Hyun Kim  ;  Hyukmin Lee  ;  Kyungwon Lee 
Citation
 JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, Vol.66(12) : 2791-2796, 2011 
Journal Title
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
ISSN
 0305-7453 
Issue Date
2011
MeSH
Cluster Analysis ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Electrophoresis, Gel, Pulsed-Field ; Genotype ; Humans ; Molecular Epidemiology ; Molecular Sequence Data ; Multilocus Sequence Typing ; Polymerase Chain Reaction ; Pseudomonas Infections/epidemiology* ; Pseudomonas Infections/microbiology* ; Pseudomonas aeruginosa/classification* ; Pseudomonas aeruginosa/enzymology* ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/isolation & purification ; Republic of Korea/epidemiology ; Sequence Analysis, DNA ; beta-Lactamases/genetics ; beta-Lactamases/secretion*
Keywords
MBLs ; meropenem ; clonal complex 235
Abstract
OBJECTIVES: To investigate the epidemiological traits of Pseudomonas aeruginosa clinical isolates producing metallo-β-lactamases (MBLs) in Korea.

METHODS: A total of 386 non-duplicate P. aeruginosa clinical isolates were collected from Korea in 2009. Detection of MBL genes was performed by PCR. The genetic organization of class 1 integrons carrying the MBL gene cassette was investigated by PCR mapping and sequencing. The epidemiological relationships of the isolates were investigated by multilocus sequence typing and PFGE.

RESULTS: Of 386 P. aeruginosa isolates, 30 (7.8%) isolates carried the bla(IMP-6) gene and 1 (0.3%) isolate carried the bla(VIM-2) gene. A probe specific for the bla(IMP-6) gene was hybridized to an ∼950 kbp I-CeuI-macrorestriction fragment from all 30 isolates and a probe specific for the bla(VIM-2) gene also hybridized to an ∼500 kbp I-CeuI-macrorestriction fragment from 1 isolate (BDC10). All 31 MBL-producing isolates shared an identical sequence type (ST), ST235, and they carried the same bla(OXA-50) allelic type, bla(OXA-50g). All MBL-producing isolates showed similar XbaI-macrorestriction patterns (similarity >85%), irrespective of MBL genotype.

CONCLUSIONS: P. aeruginosa ST235 carrying the chromosomally located bla(IMP-6) gene is widely disseminated in Korea
Files in This Item:
T201104044.pdf Download
DOI
10.1093/jac/dkr381
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94629
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