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Leukotriene B4 receptor BLT-mediated phosphorylation of NF-κB and CREB is involved in IL-8 production in human mast cells induced by Trichomonas vaginalis-derived secretory products

 Young Hee Nam  ;  Deulle Min  ;  Hyoung-Pyo Kim  ;  Kyoung-Ju Song  ;  Kyeong Ah Kim  ;  Young Ah Lee  ;  Seong Hoon Kim  ;  Myeong Heon Shin 
 MICROBES AND INFECTION, Vol.13(14-15) : 1211-1220, 2011 
Journal Title
Issue Date
Cells, Cultured ; Culture Media, Conditioned/pharmacology ; Cyclic AMP Response Element-Binding Protein/antagonists & inhibitors ; Cyclic AMP Response Element-Binding Protein/immunology* ; Cyclic AMP Response Element-Binding Protein/metabolism ; Cyclopentanes/pharmacology ; Endopeptidase K/metabolism ; Female ; Gene Expression Regulation/immunology ; Hot Temperature ; Humans ; Interleukin-8/biosynthesis ; Interleukin-8/immunology ; Leukotriene B4/immunology ; Leukotriene B4/pharmacology* ; Leukotriene B4/secretion ; Lipase/metabolism ; Mast Cells/drug effects* ; Mast Cells/immunology ; Mast Cells/metabolism ; NF-kappa B/antagonists & inhibitors ; NF-kappa B/immunology* ; NF-kappa B/metabolism ; Phosphorylation/immunology ; Receptors, Cell Surface/antagonists & inhibitors ; Receptors, Cell Surface/immunology ; Signal Transduction/immunology ; Thiosemicarbazones/pharmacology ; Transcription Factor RelA/antagonists & inhibitors ; Transcription Factor RelA/immunology ; Transcription Factor RelA/metabolism ; Trichomonas Infections/immunology* ; Trichomonas Infections/parasitology ; Trichomonas vaginalis/immunology* ; Trichomonas vaginalis/metabolism
Trichomonas vaginalis ; Mast cells ; Leukotriene B4 ; receptor BLT ; IL-8 ; NF-κB ; CREB
Trichomonas vaginalis is a protozoan parasite that causes acute tissue inflammation in vaginal trichomoniasis. In this study, we investigated the signaling mechanisms through which T. vaginalis-derived secretory products (TvSP) induce chemokine IL-8 production in human mast cells. Stimulation with TvSP induced up-regulation of IL-8 protein secretion in HMC-1 cells. In addition, TvSP induced phosphorylation of transcription factors NF-κB and CREB in HMC-1 cells. Pretreatment of TvSP with lipase, but not heat or proteinase K strongly abolished the stimulatory effect on IL-8 production. Moreover, TvSP-induced IL-8 production and phosphorylation of NF-κB or CREB were inhibited when HMC-1 cells were stimulated with modified TvSP collected from 5-lipooxygenase inhibitor-treated trichomonads. Indeed, T. vaginalis-secreted lipid mediator LTB(4) (700pg/ml) from 1×10(7) trichomonads. Furthermore, pretreatment of HMC-1 cells with antagonists for LTB(4) receptors BLT1 or BLT2 abolished the stimulatory effects of TvSP. Finally, TvSP-induced IL-8 production was inhibited by pretreatment with IκB or CREB inhibitors. These results suggest that T. vaginalis-derived secretory lipid mediator LTB(4) induces IL-8 production in mast cells via BLT-dependent activation of NF-κB and CREB.
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1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Ah(김경아)
Kim, Hyoung Pyo(김형표) ORCID logo https://orcid.org/0000-0003-1441-8822
Nam, Young Hee(남영희)
Min, A Rim(민아림) ORCID logo https://orcid.org/0000-0003-2938-9630
Song, Kyoung Ju(송경주)
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Lee, Young Ah(이영아) ORCID logo https://orcid.org/0000-0002-0414-842X
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