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Hoxc8 downregulates Mgl1 tumor suppressor gene expression and reduces its concomitant function on cell adhesion

DC Field Value Language
dc.contributor.author가디죠게스-
dc.contributor.author김명희-
dc.contributor.author이지연-
dc.date.accessioned2014-12-20T17:21:24Z-
dc.date.available2014-12-20T17:21:24Z-
dc.date.issued2011-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/94447-
dc.description.abstractHoxc8 is a homeobox gene family member, which is essential for growth and differentiation. Mgl1, a mouse homologue of the Drosophila tumor suppressor gene lgl, was previously identified as a possible target of Hoxc8. However, the biological effects and underlying molecular mechanism of Hoxc8 regulation on Mgl1 has not been fully established. The endogenous expression patterns of Hoxc8 were inversely correlated with those of Mgl1 in different types of cells and tissues. Here we showed that Hoxc8 overexpression downregulated the Mgl1 mRNA expression. Characterization of the ~2 kb Mgl1 promoter region revealed that the upstream sequence contains several putative Hox core binding sites and chromatin immunoprecipitation assay confirmed that Hoxc8 directly binds to the 5' upstream region of Mgl1. The promoter activity of this region was diminished by Hoxc8 expression but resumed by knockdown of Hoxc8 using siRNA against Hoxc8. Functional study of Mgl1 in C3H10T1/2 cells revealed a significant reduction in cell adhesion upon expression of Hoxc8. Taken together, our data suggest that Hoxc8 downregulates Mgl1 expression via direct binding to the promoter region, which in turn reduces cell adhesion and concomitant cell migration.-
dc.description.statementOfResponsibilityopen-
dc.format.extent273~279-
dc.relation.isPartOfMOLECULES AND CELLS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAsialoglycoproteins*/chemistry-
dc.subject.MESHAsialoglycoproteins*/genetics-
dc.subject.MESHAsialoglycoproteins*/metabolism-
dc.subject.MESHBase Sequence-
dc.subject.MESHCell Adhesion/drug effects-
dc.subject.MESHCell Adhesion/genetics-
dc.subject.MESHCell Movement/drug effects-
dc.subject.MESHCell Movement/genetics-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChromatin Immunoprecipitation-
dc.subject.MESHFemale-
dc.subject.MESHFibroblasts/cytology-
dc.subject.MESHFibroblasts/metabolism*-
dc.subject.MESHGene Expression Regulation, Developmental*-
dc.subject.MESHGene Silencing/drug effects-
dc.subject.MESHHomeodomain Proteins/antagonists & inhibitors-
dc.subject.MESHHomeodomain Proteins/genetics-
dc.subject.MESHHomeodomain Proteins/metabolism*-
dc.subject.MESHLectins, C-Type*/chemistry-
dc.subject.MESHLectins, C-Type*/genetics-
dc.subject.MESHLectins, C-Type*/metabolism-
dc.subject.MESHMale-
dc.subject.MESHMembrane Proteins*/chemistry-
dc.subject.MESHMembrane Proteins*/genetics-
dc.subject.MESHMembrane Proteins*/metabolism-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred ICR-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHProtein Binding-
dc.subject.MESHRNA, Small Interfering/pharmacology-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHTranscription, Genetic-
dc.titleHoxc8 downregulates Mgl1 tumor suppressor gene expression and reduces its concomitant function on cell adhesion-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentInstitute of Endocrinology (내분비연구소)-
dc.contributor.googleauthorKalyani Ruthala-
dc.contributor.googleauthorJogeswar Gadi-
dc.contributor.googleauthorJi-Yeon Lee-
dc.contributor.googleauthorHeejei Yoon-
dc.contributor.googleauthorHyun Joo Chung-
dc.contributor.googleauthorMyoung Hee Kim-
dc.identifier.doi10.1007/s10059-011-0069-8-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00001-
dc.contributor.localIdA00432-
dc.contributor.localIdA03194-
dc.relation.journalcodeJ02273-
dc.identifier.eissn0219-1032-
dc.identifier.pmid21773674-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs10059-011-0069-8-
dc.subject.keywordcell adhesion-
dc.subject.keyworddirect downstream target gene-
dc.subject.keywordHoxc8-
dc.subject.keywordMgl1-
dc.subject.keywordtumor suppressor-
dc.contributor.alternativeNameGadi, Jogeswar-
dc.contributor.alternativeNameKim, Myoung Hee-
dc.contributor.alternativeNameLee, Ji Yeon-
dc.contributor.affiliatedAuthorGadi, Jogeswar-
dc.contributor.affiliatedAuthorKim, Myoung Hee-
dc.contributor.affiliatedAuthorLee, Ji Yeon-
dc.rights.accessRightsnot free-
dc.citation.volume32-
dc.citation.number3-
dc.citation.startPage273-
dc.citation.endPage279-
dc.identifier.bibliographicCitationMOLECULES AND CELLS, Vol.32(3) : 273-279, 2011-
dc.identifier.rimsid27334-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers

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