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Cotransplantation of mouse neural stem cells (mNSCs) with adipose tissue-derived mesenchymal stem cells improves mNSC survival in a rat spinal cord injury model.

 Oh, Jin Soo  ;  Kim, Keung Nyun  ;  Ha, Yoon  ;  Choi, Byung Hyune  ;  Lim, Mi Hyun  ;  Yoon, Do Heum  ;  Gwak, So-Jung  ;  Kim, Hyo Jin  ;  Pennant, William A.  ;  An, Sung Su 
 Cell Transplantation, Vol.20(6) : 837-849, 2011 
Journal Title
 Cell Transplantation 
Issue Date
The low survival rate of graft stem cells after transplantation into recipient tissue is a major obstacle for successful stem cell therapy. After transplantation into the site of spinal cord injury, the stem cells face not only hypoxia due to low oxygen conditions, but also a lack of nutrients caused by damaged tissues and poor vascular supply. To improve the survival of therapeutic stem cells after grafting into the injured spinal cord, we examined the effects of cotransplanting mouse neural stem cells (mNSCs) and adipose tissue-derived mesenchymal stem cells (AT-MSCs) on mNSC viability. The viability of mNSCs in coculture with AT-MSCs was significantly increased compared to mNSCs alone in an in vitro injury model using serum deprivation (SD), hydrogen peroxide (H(2)O(2)), and combined (SD + H(2)O(2)) injury mimicking the ischemic environment of the injured spinal cord. We demonstrated that AT-MSCs inhibited the apoptosis of mNSCs in SD, H(2)O(2), and combined injury models. Consistent with these in vitro results, mNSCs transplanted into rat spinal cords with AT-MSCs showed better survival rates than mNSCs transplanted alone. These findings suggest that cotransplantation of mNSCs with AT-MSCs may be a more effective transplantation protocol to improve the survival of cells transplanted into the injured spinal cord.
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터)
Yonsei Authors
곽소정(Gwak, So Jung) ; 김긍년(Kim, Keung Nyun) ; 김효진(Kim, Hyo Jin) ; 안성수(An, Sung Su) ; 오진수(Oh, Jin Soo) ; 윤도흠(Yoon, Do Heum) ; 하윤(Ha, Yoon)
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