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Tracking of mitochondrial transports using a particle filtering method with a spatial constraint

Authors
 Sungmin Hong ; Hackjoon Shim ; Yoojin Chung 
Citation
 Optical Engineering, Vol.50(9) : 203~209, 2011 
Journal Title
 Optical Engineering 
ISSN
 0091-3286 
Issue Date
2011
Abstract
This paper describes a tracking method to trace the movements of fluorescently labeled mitochondria in time-lapse image sequences. It is based on particle filtering, which is a state-of-the-art tracking method, and is enhanced with a spatial constraint to improve robustness. Since mitochondria move only through axons, the spatial constraint is generated by axon segmentation on a single frame, which is the average of all the frames. The spatial constraint limits the search space of the state vector and, consequently, lowers the chance for the tracking to get lost. Using a background subtraction algorithm, the proposed method is also equipped with automatic detection of starting points, thus minimizing the requirement for user input. Implementation of the proposed method for tracking of fluorescently labeled mitochondria in time-lapse images showed substantially improved robustness and speed compared to a conventional method. With these improvements, this new particle tracking method is expected to increase the throughput of fluorescently labeled mitochondrial transport experiments, which are required for neuroscience research.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94003
DOI
10.1117/1.3629527
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Yonsei Cardiovascular Research Institute
Yonsei Authors
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Link
 http://opticalengineering.spiedigitallibrary.org/article.aspx?articleid=1158373#Abstract
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