127 191

Cited 22 times in

Improved rapid molecular diagnosis of multidrug-resistant tuberculosis using a new reverse hybridization assay, REBA MTB-MDR

 Hyeeun Bang  ;  Sangjung Park  ;  Joohwan Hwang  ;  Hyunwoo Jin  ;  Eunjin Cho  ;  Dae Yoon Kim  ;  Taeksun Song  ;  Isdore Chola Shamputa  ;  Laura E. Via  ;  Clifton E. Barry, III  ;  Sang-Nae Cho  ;  Hyeyoung Lee 
 JOURNAL OF MEDICAL MICROBIOLOGY, Vol.60(pt 10) : 1447-1454, 2011 
Journal Title
Issue Date
Antitubercular Agents/pharmacology ; Drug Resistance, Multiple, Bacterial* ; Humans ; Microbial Sensitivity Tests/methods ; Molecular Diagnostic Techniques/methods* ; Mycobacterium tuberculosis/drug effects* ; Mycobacterium tuberculosis/genetics* ; Mycobacterium tuberculosis/isolation & purification ; Nucleic Acid Hybridization/methods* ; Sensitivity and Specificity ; Tuberculosis, Multidrug-Resistant/diagnosis*
Rapid diagnosis of multidrug-resistant tuberculosis (MDR-TB) is essential for the prompt initiation of effective second-line therapy to improve treatment outcome and limit transmission of this obstinate disease. A variety of molecular methods that enable the rapid detection of mutations implicated in MDR-TB have been developed. The sensitivity of the methods is dependent, in principle, on the repertoire of mutations being detected, which is typically limited to mutations in the genes rpoB, katG and the promoter region of inhA. In this study, a new reverse hybridization assay, REBA MTB-MDR (M&D), that probes mutations in the oxyR-ahpC intergenic region, in addition to those in rpoB, katG and the inhA promoter region, was evaluated. A set of 240 Mycobacterium tuberculosis clinical isolates from patients receiving retreatment regimens was subjected to conventional phenotypic drug-susceptibility testing (DST) and the REBA MTB-MDR assay. The nucleotide sequences of the loci known to be involved in drug resistance were determined for comparison. In brief, the results showed that the REBA MTB-MDR assay efficiently recognized nucleotide changes in the oxyR-ahpC intergenic region as well as those in rpoB, katG and the inhA promoter region with higher sensitivity, resulting in an 81.0 % detection rate for isoniazid resistance. Inclusion of the oxyR-ahpC intergenic region in the REBA MTB-MDR assay improved the overall sensitivity of molecular DST for MDR-TB from 73.1 to 79.9 %.
Files in This Item:
T201103108.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sang Nae(조상래)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.