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Adenovirus-relaxin gene therapy for keloids: implication for reversing pathological fibrosis

Authors
 W.J. Lee  ;  Y.O. Kim  ;  I.K. Choi  ;  D.K. Rah  ;  C-O. Yun 
Citation
 BRITISH JOURNAL OF DERMATOLOGY, Vol.165(3) : 673-677, 2011 
Journal Title
 BRITISH JOURNAL OF DERMATOLOGY 
ISSN
 0007-0963 
Issue Date
2011
MeSH
Adenoviridae/genetics* ; Adult ; Collagen Type I/metabolism* ; Collagen Type III/metabolism* ; Enzyme-Linked Immunosorbent Assay ; Female ; Fibroblasts/metabolism ; Fibrosis/prevention & control ; Genetic Therapy/methods* ; Humans ; Immunohistochemistry ; Keloid/metabolism ; Keloid/therapy* ; Middle Aged ; Relaxin/genetics* ; Transduction, Genetic
Abstract
BACKGROUND: Keloids or hypertrophic scars are pathological proliferations of the dermal skin layer resulting from excessive collagen deposition. Because the hormone relaxin (RLX) inhibits collagen synthesis and expression in stimulated fibroblasts, an adenovirus expressing RLX (dE1-RGD/lacZ/RLX) was generated. OBJECTIVES: To investigate the effect of RLX-expressing adenovirus on expression of various extracellular matrix (ECM) components in primary keloid spheroids. METHODS: The expression levels of type I and III collagen, fibronectin and elastin were investigated by immunohistochemistry in primary keloid spheroids transduced with the RLX-expressing adenovirus. RESULTS: Immunohistochemical analysis showed that expression of major ECM components (e.g. type I and III collagen, elastin and fibronectin) was markedly reduced in primary keloid spheroids transduced with dE1-RGD/lacZ/RLX. CONCLUSIONS: These results suggest that the antifibrotic effect of RLX-expressing adenovirus may have therapeutic effects on keloids by reversing pathological fibrosis and preventing keloid recurrence after surgical excision.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2133.2011.10439.x/abstract
DOI
10.1111/j.1365-2133.2011.10439.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yong Oock(김용욱) ORCID logo https://orcid.org/0000-0002-3756-4809
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93885
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