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The gene expression profile of matrix metalloproteinases and their inhibitors in children with Henoch-Schönlein purpura

Authors
 J.I. Shin  ;  K.S. Song  ;  H. Kim  ;  N.H. Cho  ;  J. Kim  ;  H.S. Kim  ;  J.S. Lee 
Citation
 British Journal of Dermatology, Vol.164(6) : 1348-1355, 2011 
Journal Title
 British Journal of Dermatology 
ISSN
 0007-0963 
Issue Date
2011
Abstract
BACKGROUND: Because inflammatory cytokines are known to be potent inducers of matrix metalloproteinases (MMPs), and MMPs themselves can promote inflammation, we speculated that MMP activation might be involved in the pathogenesis of Henoch-Schönlein purpura (HSP) vasculitis. OBJECTIVES: To investigate the gene expression profile of all known MMPs and tissue inhibitors of metalloproteinases (TIMPs) in children with HSP and to examine the role, if any, of MMPs in the pathogenesis of HSP. METHODS: Peripheral blood samples were obtained from 10 patients with HSP (nine were in the acute stage, one had HSP nephritis) and four healthy controls. Peripheral blood samples were also taken from the nine patients with HSP when they reached the convalescent stage of the disease. From these samples, total RNA was purified and gene expressions were measured using real-time polymerase chain reaction. Results: MMP-8 expression was decreased in patients with arthralgia (P = 0·038), and MMP-3 (P = 0·03) and TIMP-4 expressions (P = 0·016) were elevated in HSP patients with nephritis. Soft tissue oedema was associated with decreased expressions of MMP-26 (P = 0·038) and MMP-28 (P = 0·038). MMP-1, MMP-8, MMP-9, MMP-10, MMP-13, MMP-16 and MMP-26 levels were significantly higher in patients in the acute stage of HSP than in normal controls (P < 0·05). MMP-9 (P = 0·097) and MMP-19 (P = 0·054) levels decreased to borderline significance in patients in the convalescent stage compared with those in the acute stage. The duration of steroid administration was negatively correlated with MMP-1, MMP-2, MMP-7, MMP-10, MMP-12, MMP-19, MMP-23 and TIMP-1 levels (P < 0·05), suggesting a suppressive effect of steroids on the expressions of MMPs and TIMPs. CONCLUSIONS: This is the first study to describe the expression profile of all known MMPs and TIMPs in children with HSP, and our results suggested that abnormal levels of MMP and TIMP activity may have a role in the pathogenesis of HSP.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93795
DOI
10.1111/j.1365-2133.2011.10295.x
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실)
Yonsei Authors
김종선(Kim, Jong Sun) ; 김호근(Kim, Ho Keun) ; 송경순(Song, Kyung Soon) ; 신재일(Shin, Jae Il) ; 이재승(Lee, Jae Seung) ; 조남훈(Cho, Nam Hoon)
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http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2133.2011.10295.x/abstract;jsessionid=151988B6AE389E16E168F914794F8601.f02t02
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