Cited 1 times in
Matrix metalloproteinase-1 induces cleavage of exogenous alphaB-crystallin transduced by a cell-penetrating peptide
DC Field | Value | Language |
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dc.contributor.author | 강석민 | - |
dc.contributor.author | 김수혁 | - |
dc.contributor.author | 정지형 | - |
dc.contributor.author | 최은영 | - |
dc.date.accessioned | 2014-12-20T16:52:09Z | - |
dc.date.available | 2014-12-20T16:52:09Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93532 | - |
dc.description.abstract | Cell-penetrating peptides (CPPs), including TAT-CPP, have been used to deliver exogenous proteins into living cells. Although a number of proteins fused to TAT-CPP can be delivered into various cells, little is known about the proteolytic cleavage of TAT-fusion proteins in cells. In this study, we demonstrate that a small heat shock protein (sHSP), alphaB-crystallin (αB-crystallin), delivered by TAT-CPP is susceptible to proteolytic cleavage by matrix metalloproteinase-1 (MMP-1) in cardiac myoblast H9c2 cells. Recombinant TAT-αB-crystallin was efficiently transduced into H9c2 cells. For a few hours following protein transduction, generation of a 14-kDa fragment, a cleavage band of TAT-αB-crystallin, increased in a time-dependent manner. This fragment was observed only in detergent-insoluble fractions. Interestingly, treatment with MMP inhibitors blocked the cleavage of TAT-αB-crystallin. In test tubes, recombinant MMP-1 processed TAT-αB-crystallin to generate the major cleavage fragment 14-kDa, as observed in the cells treated with TAT-αB-crystallin. The N-terminal sequences of the 14-kDa fragment were identified as Leu-Arg-Ala-Pro-Ser-Trp-Phe, indicating that this fragment is generated by cleavage at Phe54-Leu55 of αB-crystallin. The MMP-1-selective inhibitor abolished the production of 14-kDa fragments in cells. In addition, the cleaved fragment of TAT-αB-crystallin was significantly reduced in cells transfected with MMP-1 siRNA. Moreover, the enzymatic activity of MMP-1 was markedly increased in TAT-αB-crystallin-treated cells. TAT-αB-crystallin has a cytoprotective effect on H9c2 cells under hypoxic insult, moreover, MMP-1-selective inhibitor treatment led to even increased cell viability. These results suggest that MMP-1 is responsible for proteolytic cleavage of TAT-αB-crystallin during its intracellular transduction in H9c2 cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2454~2462 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Hypoxia | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | Cell-Penetrating Peptides/isolation & purification | - |
dc.subject.MESH | Cell-Penetrating Peptides/pharmacokinetics* | - |
dc.subject.MESH | Cell-Penetrating Peptides/pharmacology | - |
dc.subject.MESH | Cytoprotection | - |
dc.subject.MESH | Drug Delivery Systems | - |
dc.subject.MESH | Enzyme Assays | - |
dc.subject.MESH | Matrix Metalloproteinase 1/genetics | - |
dc.subject.MESH | Matrix Metalloproteinase 1/metabolism* | - |
dc.subject.MESH | Myoblasts/drug effects | - |
dc.subject.MESH | Myoblasts/enzymology | - |
dc.subject.MESH | Myoblasts/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Recombinant Fusion Proteins/isolation & purification | - |
dc.subject.MESH | Recombinant Fusion Proteins/pharmacokinetics* | - |
dc.subject.MESH | Recombinant Fusion Proteins/pharmacology | - |
dc.subject.MESH | alpha-Crystallin B Chain/isolation & purification | - |
dc.subject.MESH | alpha-Crystallin B Chain/pharmacokinetics* | - |
dc.subject.MESH | alpha-Crystallin B Chain/pharmacology | - |
dc.subject.MESH | tat Gene Products, Human Immunodeficiency Virus/isolation & purification | - |
dc.subject.MESH | tat Gene Products, Human Immunodeficiency Virus/pharmacokinetics* | - |
dc.subject.MESH | tat Gene Products, Human Immunodeficiency Virus/pharmacology | - |
dc.title | Matrix metalloproteinase-1 induces cleavage of exogenous alphaB-crystallin transduced by a cell-penetrating peptide | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Research Institute for Cerebral & Cardiovascular Diseases (심혈관제품유효성평가센터) | - |
dc.contributor.googleauthor | Seung Won Yang | - |
dc.contributor.googleauthor | Seung-Min Lee | - |
dc.contributor.googleauthor | Eun Young Choi | - |
dc.contributor.googleauthor | Kyung Hye Lee | - |
dc.contributor.googleauthor | Soo Hyuk Kim | - |
dc.contributor.googleauthor | Min-Jeong Shin | - |
dc.contributor.googleauthor | Ye Sun Han | - |
dc.contributor.googleauthor | Seok-Min Kang | - |
dc.contributor.googleauthor | Ji Hyung Chung | - |
dc.identifier.doi | 10.1002/jcb.23167 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00037 | - |
dc.contributor.localId | A00640 | - |
dc.contributor.localId | A03739 | - |
dc.contributor.localId | A04154 | - |
dc.relation.journalcode | J01303 | - |
dc.identifier.eissn | 1097-4644 | - |
dc.identifier.pmid | 21538481 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jcb.23167/abstract | - |
dc.subject.keyword | Alphab‐crystallin | - |
dc.subject.keyword | Cell‐penetrating peptide | - |
dc.subject.keyword | MMP‐1 | - |
dc.subject.keyword | Proteolysis | - |
dc.contributor.alternativeName | Kang, Seok Min | - |
dc.contributor.alternativeName | Kim, Soo Hyuk | - |
dc.contributor.alternativeName | Chung, Ji Hyung | - |
dc.contributor.alternativeName | Choi, Eun Young | - |
dc.contributor.affiliatedAuthor | Kang, Seok Min | - |
dc.contributor.affiliatedAuthor | Kim, Soo Hyuk | - |
dc.contributor.affiliatedAuthor | Chung, Ji Hyung | - |
dc.contributor.affiliatedAuthor | Choi, Eun Young | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 112 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2454 | - |
dc.citation.endPage | 2462 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, Vol.112(9) : 2454-2462, 2011 | - |
dc.identifier.rimsid | 28290 | - |
dc.type.rims | ART | - |
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