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Delphinidin, a specific inhibitor of histone acetyltransferase, suppresses inflammatory signaling via prevention of NF-κB acetylation in fibroblast-like synoviocyte MH7A cells.

Authors
 Ah-Reum Seong  ;  Jung-Yoon Yoo  ;  KyungChul Choi  ;  Mee-Hee Lee  ;  Yoo-Hyun Lee  ;  Jeongmin Lee  ;  Woojin Jun  ;  Sunoh Kim  ;  Ho-Geun Yoon 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.410(3) : 581-586, 2011 
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
 0006-291X 
Issue Date
2011
MeSH
Acetylation/drug effects ; Anthocyanins/pharmacology* ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology* ; Arthritis/genetics ; Arthritis/immunology* ; Cell Line ; Cytokines/antagonists & inhibitors* ; Enzyme Inhibitors/pharmacology* ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Gene Expression/drug effects ; Gene Expression/genetics ; Histone Acetyltransferases/antagonists & inhibitors* ; Humans ; Jurkat Cells ; NF-kappa B/metabolism* ; Synovial Fluid/drug effects ; Synovial Fluid/metabolism
Keywords
Dephinidin ; Histone acetyltransferase inhibitor ; Inflammation ; NF-κB ; Cytokines ; Rheumatoid arthritis
Abstract
Histone acetyltransferase (HAT) inhibitors (HATi) isolated from dietary compounds have been shown to suppress inflammatory signaling, which contributes to rheumatoid arthritis. Here, we identified a novel HATi in Punica granatum L. known as delphinidin (DP). DP did not affect the activity of other epigenetic enzymes (histone deacetylase, histone methyltransferase, or sirtuin1). DP specifically inhibited the HAT activities of p300/CBP. It also inhibited p65 acetylation in MH7A cells, a human rheumatoid arthritis synovial cell line. DP-induced hypoacetylation was accompanied by cytosolic accumulation of p65 and nuclear localization of IKBα. Accordingly, DP treatment inhibited TNFα-stimulated increases in NF-κB function and expression of NF-κB target genes in these cells. Importantly, DP suppressed lipopolysaccharide-induced pro-inflammatory cytokine expression in Jurkat T lymphocytes, demonstrating that HATi efficiently suppresses cytokine-mediated immune responses. Together, these results show that the HATi activity of DP counters anti-inflammatory signaling by blocking p65 acetylation and that this compound may be useful in preventing inflammatory arthritis.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X11009879
DOI
10.1016/j.bbrc.2011.06.029
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Seong, Ah Reum(성아름)
Yoo, Jung Yoon(유정윤) ORCID logo https://orcid.org/0000-0001-9366-3863
Yoon, Ho Geun(윤호근) ORCID logo https://orcid.org/0000-0003-2718-3372
Choi, Kyung Chul(최경철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93501
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