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Sensitive angiogenesis imaging of orthotopic bladder tumors in mice using a selective magnetic resonance imaging contrast agent containing VEGF121/rGel

DC Field Value Language
dc.contributor.author서진석-
dc.contributor.author양재문-
dc.contributor.author허용민-
dc.date.accessioned2014-12-20T16:49:15Z-
dc.date.available2014-12-20T16:49:15Z-
dc.date.issued2011-
dc.identifier.issn0020-9996-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/93440-
dc.description.abstractOBJECTIVES: To investigate the efficiency of magnetic resonance imaging (MRI) contrast agents employing vascular endothelial growth factor (VEGF121)/rGel conjugated MnFe2O4 nanocrystals for imaging of neovasculature using a bladder tumor model. MATERIALS AND METHODS: VEGF121/rGel was conjugated to MnFe2O4 nanoparticles (MNPs). The targeting efficiency and detection capability of the VEGF121/rGel-MNPs were investigated in both KDR-deficient (253JB-V) and KDR-overexpressing (PAE/KDR) cells using MRI. The internalization of VEGF121/rGel-MNPs into cells was confirmed by electron microscopy. Their phosphorylation ability and cytotoxicity were compared with unconjugated VEGF121/rGel. The orthotopic tumor mice were established by implanting low KDR-expressing 253JB-V cells into the bladder dome. After tail-vein injection of VEGF121/rGel-MNPs, the MR signal enhancement of intratumoral vessels by VEGF121/rGel-MNPs was observed and inhibition test using VEGF121 was also conducted. Ex vivo MR imaging of tumor tissue, and a fluorescence immunostaining study was also performed. RESULTS: The water-soluble VEGF121/rGel-MNPs (44.5 ± 1.2 nm) were stably suspended in the biologic media and exhibited a high relaxivity coefficient (423 mMs). They demonstrated sufficient targeting capability against KDR-overexpressing PAE/KDR cells, as confirmed by dose-dependent MR images and VEGF121 inhibition tests. The phosphorylation activity of KDR and cytotoxicity of VEGF121/rGel-MNPs were evaluated. VEGF121/rGel-MNPs successfully targeted the tumor and provided accurate anatomic details through (i) acquisition of clear neoangiogenic vascular distributions and (ii) obvious enhancement of the MR signal in T2*-weighted images. Immunostaining and blocking studies demonstrated the specific targeting ability of VEGF121/rGel-MNPs toward intratumoral angiogenesis. CONCLUSIONS: Synthesized VEGF121/rGel-MNPs as targeted MR imaging contrast agents can be specifically delivered to tumors and bind to KDR-expressing angiogenic tumor vessels.-
dc.description.statementOfResponsibilityopen-
dc.format.extent441~449-
dc.relation.isPartOfINVESTIGATIVE RADIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHContrast Media*-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHFerric Compounds*-
dc.subject.MESHMale-
dc.subject.MESHManganese Compounds*-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHNanoconjugates*-
dc.subject.MESHNeovascularization, Pathologic-
dc.subject.MESHRibosome Inactivating Proteins, Type 1*-
dc.subject.MESHSensitivity and Specificity-
dc.subject.MESHUrinary Bladder Neoplasms/blood supply*-
dc.subject.MESHUrinary Bladder Neoplasms/diagnosis*-
dc.subject.MESHVascular Endothelial Growth Factor A*-
dc.titleSensitive angiogenesis imaging of orthotopic bladder tumors in mice using a selective magnetic resonance imaging contrast agent containing VEGF121/rGel-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Radiology (영상의학)-
dc.contributor.googleauthorEun-Jin Cho-
dc.contributor.googleauthorJaemoon Yang-
dc.contributor.googleauthorKhalid A. Mohamedali-
dc.contributor.googleauthorEun-Kyung Lim-
dc.contributor.googleauthorEun-Jung Kim-
dc.contributor.googleauthorCarol J. Farhangfar-
dc.contributor.googleauthorJin-Suck Suh-
dc.contributor.googleauthorSeungjoo Haam-
dc.contributor.googleauthorMichael G. Rosenblum-
dc.contributor.googleauthorYong-Min Huh-
dc.identifier.doi10.1097/RLI.0b013e3182174fad-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02315-
dc.contributor.localIdA01916-
dc.contributor.localIdA04359-
dc.relation.journalcodeJ01188-
dc.identifier.eissn1536-0210-
dc.identifier.pmid21512397-
dc.identifier.urlhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00004424-201107000-00005&LSLINK=80&D=ovft-
dc.subject.keywordangiogenesis-
dc.subject.keywordVEGF121/rGel-
dc.subject.keywordnanoparticles-
dc.subject.keywordmagnetic resonance-
dc.subject.keywordcontrast agent-
dc.contributor.alternativeNameSuh, Jin Suck-
dc.contributor.alternativeNameYang, Jae Moon-
dc.contributor.alternativeNameHuh, Yong Min-
dc.contributor.affiliatedAuthorYang, Jae Moon-
dc.contributor.affiliatedAuthorSuh, Jin Suck-
dc.contributor.affiliatedAuthorHuh, Yong Min-
dc.rights.accessRightsnot free-
dc.citation.volume46-
dc.citation.number7-
dc.citation.startPage441-
dc.citation.endPage449-
dc.identifier.bibliographicCitationINVESTIGATIVE RADIOLOGY, Vol.46(7) : 441-449, 2011-
dc.identifier.rimsid28235-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers

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