Cited 37 times in
Sensitive angiogenesis imaging of orthotopic bladder tumors in mice using a selective magnetic resonance imaging contrast agent containing VEGF121/rGel
DC Field | Value | Language |
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dc.contributor.author | 서진석 | - |
dc.contributor.author | 양재문 | - |
dc.contributor.author | 허용민 | - |
dc.date.accessioned | 2014-12-20T16:49:15Z | - |
dc.date.available | 2014-12-20T16:49:15Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0020-9996 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93440 | - |
dc.description.abstract | OBJECTIVES: To investigate the efficiency of magnetic resonance imaging (MRI) contrast agents employing vascular endothelial growth factor (VEGF121)/rGel conjugated MnFe2O4 nanocrystals for imaging of neovasculature using a bladder tumor model. MATERIALS AND METHODS: VEGF121/rGel was conjugated to MnFe2O4 nanoparticles (MNPs). The targeting efficiency and detection capability of the VEGF121/rGel-MNPs were investigated in both KDR-deficient (253JB-V) and KDR-overexpressing (PAE/KDR) cells using MRI. The internalization of VEGF121/rGel-MNPs into cells was confirmed by electron microscopy. Their phosphorylation ability and cytotoxicity were compared with unconjugated VEGF121/rGel. The orthotopic tumor mice were established by implanting low KDR-expressing 253JB-V cells into the bladder dome. After tail-vein injection of VEGF121/rGel-MNPs, the MR signal enhancement of intratumoral vessels by VEGF121/rGel-MNPs was observed and inhibition test using VEGF121 was also conducted. Ex vivo MR imaging of tumor tissue, and a fluorescence immunostaining study was also performed. RESULTS: The water-soluble VEGF121/rGel-MNPs (44.5 ± 1.2 nm) were stably suspended in the biologic media and exhibited a high relaxivity coefficient (423 mMs). They demonstrated sufficient targeting capability against KDR-overexpressing PAE/KDR cells, as confirmed by dose-dependent MR images and VEGF121 inhibition tests. The phosphorylation activity of KDR and cytotoxicity of VEGF121/rGel-MNPs were evaluated. VEGF121/rGel-MNPs successfully targeted the tumor and provided accurate anatomic details through (i) acquisition of clear neoangiogenic vascular distributions and (ii) obvious enhancement of the MR signal in T2*-weighted images. Immunostaining and blocking studies demonstrated the specific targeting ability of VEGF121/rGel-MNPs toward intratumoral angiogenesis. CONCLUSIONS: Synthesized VEGF121/rGel-MNPs as targeted MR imaging contrast agents can be specifically delivered to tumors and bind to KDR-expressing angiogenic tumor vessels. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 441~449 | - |
dc.relation.isPartOf | INVESTIGATIVE RADIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Contrast Media* | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Ferric Compounds* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Manganese Compounds* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Nanoconjugates* | - |
dc.subject.MESH | Neovascularization, Pathologic | - |
dc.subject.MESH | Ribosome Inactivating Proteins, Type 1* | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Urinary Bladder Neoplasms/blood supply* | - |
dc.subject.MESH | Urinary Bladder Neoplasms/diagnosis* | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A* | - |
dc.title | Sensitive angiogenesis imaging of orthotopic bladder tumors in mice using a selective magnetic resonance imaging contrast agent containing VEGF121/rGel | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiology (영상의학) | - |
dc.contributor.googleauthor | Eun-Jin Cho | - |
dc.contributor.googleauthor | Jaemoon Yang | - |
dc.contributor.googleauthor | Khalid A. Mohamedali | - |
dc.contributor.googleauthor | Eun-Kyung Lim | - |
dc.contributor.googleauthor | Eun-Jung Kim | - |
dc.contributor.googleauthor | Carol J. Farhangfar | - |
dc.contributor.googleauthor | Jin-Suck Suh | - |
dc.contributor.googleauthor | Seungjoo Haam | - |
dc.contributor.googleauthor | Michael G. Rosenblum | - |
dc.contributor.googleauthor | Yong-Min Huh | - |
dc.identifier.doi | 10.1097/RLI.0b013e3182174fad | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02315 | - |
dc.contributor.localId | A01916 | - |
dc.contributor.localId | A04359 | - |
dc.relation.journalcode | J01188 | - |
dc.identifier.eissn | 1536-0210 | - |
dc.identifier.pmid | 21512397 | - |
dc.identifier.url | http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00004424-201107000-00005&LSLINK=80&D=ovft | - |
dc.subject.keyword | angiogenesis | - |
dc.subject.keyword | VEGF121/rGel | - |
dc.subject.keyword | nanoparticles | - |
dc.subject.keyword | magnetic resonance | - |
dc.subject.keyword | contrast agent | - |
dc.contributor.alternativeName | Suh, Jin Suck | - |
dc.contributor.alternativeName | Yang, Jae Moon | - |
dc.contributor.alternativeName | Huh, Yong Min | - |
dc.contributor.affiliatedAuthor | Yang, Jae Moon | - |
dc.contributor.affiliatedAuthor | Suh, Jin Suck | - |
dc.contributor.affiliatedAuthor | Huh, Yong Min | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 46 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 441 | - |
dc.citation.endPage | 449 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE RADIOLOGY, Vol.46(7) : 441-449, 2011 | - |
dc.identifier.rimsid | 28235 | - |
dc.type.rims | ART | - |
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