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Multiple target loci assembly sequencing (mTAS).

Authors
 Hyojun Han  ;  Jung-ki Yoon  ;  Byoung Chul Cho  ;  Hwangbeom Kim  ;  Duhee Bang 
Citation
 ANALYTICAL BIOCHEMISTRY, Vol.415(2) : 218-220, 2011 
Journal Title
ANALYTICAL BIOCHEMISTRY
ISSN
 0003-2697 
Issue Date
2011
MeSH
Exons ; GeneticLoci* ; Genome, Human ; Humans ; Polymerase Chain Reaction/methods ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA/methods*
Abstract
Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis.
Full Text
http://www.sciencedirect.com/science/article/pii/S0003269711002375
DOI
10.1016/j.ab.2011.04.012
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Cho, Byoung Chul(조병철) ORCID logo https://orcid.org/0000-0002-5562-270X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93375
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