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Multiple target loci assembly sequencing (mTAS).
DC Field | Value | Language |
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dc.contributor.author | 조병철 | - |
dc.date.accessioned | 2014-12-20T16:47:11Z | - |
dc.date.available | 2014-12-20T16:47:11Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93375 | - |
dc.description.abstract | Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 218~220 | - |
dc.relation.isPartOf | ANALYTICAL BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Exons | - |
dc.subject.MESH | GeneticLoci* | - |
dc.subject.MESH | Genome, Human | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Polymerase Chain Reaction/methods | - |
dc.subject.MESH | Polymorphism, Single Nucleotide | - |
dc.subject.MESH | Sequence Analysis, DNA/methods* | - |
dc.title | Multiple target loci assembly sequencing (mTAS). | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Hyojun Han | - |
dc.contributor.googleauthor | Jung-ki Yoon | - |
dc.contributor.googleauthor | Byoung Chul Cho | - |
dc.contributor.googleauthor | Hwangbeom Kim | - |
dc.contributor.googleauthor | Duhee Bang | - |
dc.identifier.doi | 10.1016/j.ab.2011.04.012 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03822 | - |
dc.relation.journalcode | J00135 | - |
dc.identifier.eissn | 1096-0309 | - |
dc.identifier.pmid | 21536013 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0003269711002375 | - |
dc.contributor.alternativeName | Cho, Byoung Chul | - |
dc.contributor.affiliatedAuthor | Cho, Byoung Chul | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 415 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 218 | - |
dc.citation.endPage | 220 | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, Vol.415(2) : 218-220, 2011 | - |
dc.identifier.rimsid | 27173 | - |
dc.type.rims | ART | - |
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