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Lack of effect of dexamethasone on growth of Orientia tsutsugamushi Gilliam in mouse L929 cells

 Chang Oh Kim  ;  Ae Jung Huh  ;  June Myung Kim  ;  Young Goo Song  ;  Jun Yong Choi  ;  Su Jin Jeong  ;  Sang Hoon Han  ;  Bum Sik Chin  ;  Kkot Sil Lee  ;  Joon Sup Yeom 
 Yonsei Medical Journal, Vol.52(4) : 624-629, 2011 
Journal Title
 Yonsei Medical Journal 
Issue Date
PURPOSE: Previous studies and our own clinical experience suggest that concurrent corticosteroid treatment for severe rickettsial disease with multiorgan failure may improve the clinical course or reduce mortality. However, the use of corticosteroids as adjunctive treatment for rickettsial diseases is controversial. We attempted to determine the influences of corticosteroid on the growth of Orientia tsutsugamushi in vitro to justify and evaluate the clinical applicability of corticosteroid in rickettsial disease. MATERIALS AND METHODS: L929 cells were infected with Orientia tsutsugamushi Gilliam. Dexamethasone was added to the cells at final concentrations of 10¹ and 10⁷ pg/mL. Cultures were incubated at 35°C and processed for flow cytometry on the 6th day after addition of dexamethasone. RESULTS: Observation on the 6th day after treatment with dexamethasone in infected cultures revealed that there was no difference in fluorescence intensity among the treatment wells. Treatment of the cells with dexamethasone at concentrations of 10¹ and 10⁷ pg/mL showed no influence on the growth of Orientia tsutsugamushi. CONCLUSION: Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro. Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.
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1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
Yonsei Authors
김준명(Kim, June Myung) ; 김창오(Kim, Chang Oh) ; 송영구(Song, Young Goo) ; 정수진(Jeong, Su Jin) ; 진범식(Chin, Bum Sik) ; 최준용(Choi, Jun Yong) ; 한상훈(Han, Sang Hoon)
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