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Lack of effect of dexamethasone on growth of Orientia tsutsugamushi Gilliam in mouse L929 cells

Authors
 Chang Oh Kim  ;  Ae Jung Huh  ;  Joon Sup Yeom  ;  Kkot Sil Lee  ;  Bum Sik Chin  ;  Sang Hoon Han  ;  Su Jin Jeong  ;  Jun Yong Choi  ;  Young Goo Song  ;  June Myung Kim 
Citation
 YONSEI MEDICAL JOURNAL, Vol.52(4) : 624-629, 2011 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
2011
MeSH
Animals ; Cell Line ; Cell Proliferation/drug effects ; Dexamethasone/pharmacology* ; Flow Cytometry ; Interferon-gamma/pharmacology ; Mice ; Orientia tsutsugamushi/drug effects* ; Orientia tsutsugamushi/growth & development ; Scrub Typhus/drug therapy ; Scrub Typhus/microbiology
Keywords
Orientia tsutsugamushi ; L929 cells ; corticosteroids ; interferon
Abstract
PURPOSE: Previous studies and our own clinical experience suggest that concurrent corticosteroid treatment for severe rickettsial disease with multiorgan failure may improve the clinical course or reduce mortality. However, the use of corticosteroids as adjunctive treatment for rickettsial diseases is controversial. We attempted to determine the influences of corticosteroid on the growth of Orientia tsutsugamushi in vitro to justify and evaluate the clinical applicability of corticosteroid in rickettsial disease.

MATERIALS AND METHODS: L929 cells were infected with Orientia tsutsugamushi Gilliam. Dexamethasone was added to the cells at final concentrations of 10¹ and 10⁷ pg/mL. Cultures were incubated at 35°C and processed for flow cytometry on the 6th day after addition of dexamethasone.

RESULTS: Observation on the 6th day after treatment with dexamethasone in infected cultures revealed that there was no difference in fluorescence intensity among the treatment wells. Treatment of the cells with dexamethasone at concentrations of 10¹ and 10⁷ pg/mL showed no influence on the growth of Orientia tsutsugamushi.

CONCLUSION: Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro. Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.
Files in This Item:
T201101413.pdf Download
DOI
10.3349/ymj.2011.52.4.624
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, June Myung(김준명)
Kim, Chang Oh(김창오) ORCID logo https://orcid.org/0000-0002-0773-5443
Song, Young Goo(송영구) ORCID logo https://orcid.org/0000-0002-0733-4156
Jeong, Su Jin(정수진) ORCID logo https://orcid.org/0000-0003-4025-4542
Chin, Bum Sik(진범식)
Choi, Jun Yong(최준용) ORCID logo https://orcid.org/0000-0002-2775-3315
Han, Sang Hoon(한상훈) ORCID logo https://orcid.org/0000-0002-4278-5198
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93221
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