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Genetic alterations in oral squamous cell carcinoma progression detected by combining array-based comparative genomic hybridization and multiplex ligation-dependent probe amplification

Authors
 Jeong-Dan Cha  ;  Hyung Jun Kim  ;  In-Ho Cha 
Citation
 ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, Vol.111(5) : 594-607, 2011 
Journal Title
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS
ISSN
 1079-2104 
Issue Date
2011
MeSH
Aged ; Carcinoma, Squamous Cell/genetics* ; Carcinoma, Squamous Cell/pathology ; Chromosome Aberrations ; Cluster Analysis ; Comparative Genomic Hybridization* ; DNA Copy Number Variations* ; Disease Progression ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; Middle Aged ; Mouth Mucosa/pathology ; Mouth Neoplasms/genetics* ; Mouth Neoplasms/pathology ; Neoplasm Staging ; Nucleic Acid Amplification Techniques* ; Oligonucleotide Array Sequence Analysis/methods
Abstract
BACKGROUND: Oral squamous cell carcinoma (OSCC), the most common malignancy of the oral cavity, has been shown to occur via a multistep process driven by the accumulation of carcinogen-induced genetic changes.

STUDY DESIGN: Array-based comparative genomic hybridization (aCGH) and multiplex ligation-dependent probe amplification (MLPA) were conducted to screen human genomewide alterations on fresh tissues of the cancer area, the dysplastic transitional area, and the resection margin (normal) free of tumor; these samples were obtained from 7 OSCC patients.

RESULTS: The highest amplification frequencies (100%, 7/7) were detected in FAM5B, TIPARP, PIK3CA, NLGN1, FGF10, HDAC9, GRM3, DDEF1, EDNRB, CHRDL1, and HTR2C, and the highest deletion frequencies in THRAP3, CTTNBP2NL, GATAD2B, REL, CKAP2L, RHOA, EIF4E3, PDLIM5, FBXO3, NEUROD4, and ABCA5 in the OSCC. In the dysplasia, amplification (100%, 7/7) was detected in RNF36 and deletion in CKAP2L and TCF8. We could detect large differences with MLPA in the number of alterations between the cancer or dysplasia versus the normal area with P values of <.001.

CONCLUSION: These findings indicate that these DNA copy number changes on each chromosome in the 3 categories may be associated with OSCC tumorigenesis and/or progression.
Full Text
http://www.sciencedirect.com/science/article/pii/S1079210410009200
DOI
10.1016/j.tripleo.2010.11.020
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
Yonsei Authors
Cha, In Ho(차인호) ORCID logo https://orcid.org/0000-0001-8259-2190
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93080
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