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Niflumic Acid Reduces Histamine-Induced MUC5AC Expression in Human Conjunctival Epithelial Cells

Authors
 Seo K.Y.  ;  Jeon S.  ;  Choi S.H.  ;  Chung S.-H. 
Citation
 Ophthalmic Research, Vol.46(4) : 181-186, 2011 
Journal Title
 Ophthalmic Research 
ISSN
 0030-3747 
Issue Date
2011
MeSH
Anti-Inflammatory Agents, Non-Steroidal/pharmacology* ; Blotting, Western ; Cells, Cultured ; Chloride Channels/antagonists & inhibitors ; Chloride Channels/metabolism ; Conjunctiva/cytology* ; Enzyme-Linked Immunosorbent Assay ; Epithelial Cells/drug effects* ; Epithelial Cells/metabolism ; Gene Expression Regulation/drug effects* ; Histamine/pharmacology* ; Histamine H1 Antagonists/pharmacology ; Histamine H2 Antagonists/pharmacology ; Humans ; Mucin 5AC/genetics* ; Mucin 5AC/metabolism ; Niflumic Acid/pharmacology* ; RNA, Messenger/genetics ; Real-Time Polymerase Chain Reaction ; Receptors, Histamine/genetics
Keywords
Human calcium-activated chloride channel 1 ; Histamine ; Human conjunctival epithelial cells ; MUC5AC
Abstract
Background/Aims: Histamine is an important inflammatory mediator in allergic conjunctivitis. The aim of the present study was to explore whether histamine induced MUC5AC production in human conjunctival epithelial cells (HCECs), and to describe the relationship between human calcium-activated chloride channel 1 (hCLCA1) activity and MUC5AC expression. Methods: HCECs were isolated from human conjunctiva and cultured at an air-liquid interface. MUC5AC and hCLCA1 mRNA expression was examined by real-time PCR after exposure of the cells to histamine. MUC5AC secretion was quantitated by ELISA. hCLCA1 protein was detected by Western blot analysis. Results: Histamine upregulated MUC5AC expression and MUC5AC secretion in a dose-dependent manner. Histamine also upregulated hCLCA1 mRNA expression in a dose-dependent manner. Preincubation of histamine receptor 1 antagonist reduced histamine-induced MUC5AC and hCLCA1 mRNA expression. Niflumic acid, a chloride channel inhibitor, reduced histamine-induced MUC5ACand hCLCA1 expression. Conclusion: Histamine-induced MUC5AC production could be decreased by niflumic acid, a calcium-activated chloride channel inhibitor.
Full Text
http://www.karger.com/Article/FullText/325026
DOI
10.1159/000325026
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Seo, Kyuong Yul(서경률) ORCID logo https://orcid.org/0000-0002-9855-1980
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/92936
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