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Effects of agmatine on hypoxic microglia and activity of nitric oxide synthase

DC FieldValueLanguage
dc.contributor.author박경아-
dc.contributor.author이원택-
dc.contributor.author이종은-
dc.contributor.author홍사민-
dc.contributor.author박유미-
dc.date.accessioned2014-12-20T16:25:25Z-
dc.date.available2014-12-20T16:25:25Z-
dc.date.issued2011-
dc.identifier.issn0006-8993-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/92688-
dc.description.abstractMicroglia are the resident macrophages of CNS and play a crucial role in maintaining homeostasis against various neuronal injuries. However, excessive activation of microglia may destroy healthy neurons as well as damaged neurons. We investigated neuroprotective effects of amgatine on hypoxic microglia using in vitro and in vivo models for transient hypoxia. For in vitro study, BV2 immortalized murine microglia were incubated with or without 100 μM of agmatine in a closed anaerobic chamber for 2h. After recovery in normoxic condition for 20 h, cell viability and the amount of nitrite generation were determined. For in vivo study, 100mg/kg of agmatine or equivalent volume of saline was intraperitoneally administered, and the left middle cerebral artery of adult male Sprague-Dawley rats was occluded for 90 min. After 24h from occlusion, the cortex and striatum of the forebrains was evaluated to check the immunoreactivity with a microglial marker, ionized calcium binding adaptor molecule 1 (Iba1), and inducible nitric oxide synthase (iNOS). Results showed that agmatine attenuated hypoxia-induced cytotoxicity and nitrite production by BV2 microglia. Agmatine also decreased the activities of microglia and NOS induced by transient middle cerebral artery occlusion. Finally, our findings reveal that agmatine may reduce microglial damages caused by transient hypoxia and suggest that agmatine may lead to a novel therapeutic strategy for hypoxic neuronal injuries.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfBrain Research-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAgmatine/pharmacology*-
dc.subject.MESHAgmatine/therapeutic use-
dc.subject.MESHAnalysis of Variance-
dc.subject.MESHAnimals-
dc.subject.MESHCalcium-Binding Proteins/metabolism-
dc.subject.MESHCell Hypoxia/drug effects*-
dc.subject.MESHCell Line, Transformed-
dc.subject.MESHCerebrovascular Circulation/drug effects-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHDose-Response Relationship, Drug-
dc.subject.MESHGene Expression Regulation, Enzymologic/drug effects-
dc.subject.MESHInfarction, Middle Cerebral Artery/drug therapy-
dc.subject.MESHInfarction, Middle Cerebral Artery/pathology-
dc.subject.MESHL-Lactate Dehydrogenase/metabolism-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMicrofilament Proteins-
dc.subject.MESHMicroglia/drug effects*-
dc.subject.MESHMicroglia/enzymology*-
dc.subject.MESHNitric Oxide Synthase Type II/metabolism*-
dc.subject.MESHNitrites/metabolism-
dc.subject.MESHProsencephalon/drug effects-
dc.subject.MESHProsencephalon/enzymology-
dc.subject.MESHProsencephalon/pathology-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.titleEffects of agmatine on hypoxic microglia and activity of nitric oxide synthase-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학)-
dc.contributor.googleauthorSoo Kyung Ahn-
dc.contributor.googleauthorSamin Hong-
dc.contributor.googleauthorYu Mi Park-
dc.contributor.googleauthorWon Taek Lee-
dc.contributor.googleauthorKyung Ah Park-
dc.contributor.googleauthorJong Eun Lee-
dc.identifier.doi10.1016/j.brainres.2010.12.002-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01424-
dc.contributor.localIdA03007-
dc.contributor.localIdA04395-
dc.contributor.localIdA03146-
dc.contributor.localIdA04937-
dc.relation.journalcodeJ00392-
dc.identifier.pmid21145312-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006899310026132-
dc.subject.keywordAgmatine-
dc.subject.keywordHypoxia-
dc.subject.keywordMicroglia-
dc.subject.keywordNeuroprotection-
dc.subject.keywordNitric oxide synthase-
dc.contributor.alternativeNamePark, Kyung Ah-
dc.contributor.alternativeNamePark, Yoo Mi-
dc.contributor.alternativeNameLee, Won Taek-
dc.contributor.alternativeNameLee, Jong Eun-
dc.contributor.alternativeNameHong, Sa Min-
dc.contributor.affiliatedAuthorPark, Kyung Ah-
dc.contributor.affiliatedAuthorLee, Won Taek-
dc.contributor.affiliatedAuthorHong, Sa Min-
dc.contributor.affiliatedAuthorLee, Jong Eun-
dc.contributor.affiliatedAuthorPark, Yoo Mi-
dc.rights.accessRightsnot free-
dc.citation.volume1373-
dc.citation.startPage48-
dc.citation.endPage54-
dc.identifier.bibliographicCitationBrain Research, Vol.1373 : 48-54, 2011-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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