Cited 26 times in
Down-regulation of Bcl-2 is mediated by NF-κB activation in Helicobacter pylori-induced apoptosis of gastric epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.contributor.author | 김동구 | - |
dc.contributor.author | 추상희 | - |
dc.date.accessioned | 2014-12-20T16:20:38Z | - |
dc.date.available | 2014-12-20T16:20:38Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0036-5521 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/92540 | - |
dc.description.abstract | OBJECTIVE: Bcl-2 family is involved in the regulation of apoptosis. NF-κB activation is associated with either the expression of Bcl-2 or down-regulation of Bcl-2 depending on cell types and stimuli. Previously, we showed NF-κB activation, decrease in the level of Bcl-2, and apoptosis in Helicobacter pylori (H. pylori)-infected gastric epithelial cells. The present study aims to investigate the relation of Bcl-2 expression and NF-κB activation in H. pylori-induced apoptotic cell death of AGS (gastric adenocarcinoma) cells. MATERIAL AND METHODS: AGS cells were transfected with mutant IκBα to suppress NF-κB activation or Bcl-2 gene to induce overexpression of Bcl-2. mRNA expression of Bcl-2, p53 and Bax, DNA fragmentation, cell viability, and the numbers of apoptotic cells were determined. RESULTS: H. pylori induced decrease in Bcl-2, but increase in p53 and Bax at the levels of mRNA and protein in AGS cells. H. pylori-induced increment of apoptotic cells and decrease in Bcl-2 level were inhibited in the cells transfected with mutant IκBα gene as compared with the cells transfected with control vector. H. pylori-induced apoptosis determined by apoptotic cells, DNA fragmentation, and cell viability was inhibited in the cells transfected with Bcl-2 gene. CONCLUSION: Down-regulation of Bcl-2 is mediated by NF-κB activation, which may be the underlying mechanism of apoptosis in H. pylori-infected gastric epithelial cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 148~155 | - |
dc.relation.isPartOf | SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Down-regulation of Bcl-2 is mediated by NF-κB activation in Helicobacter pylori-induced apoptosis of gastric epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Sang Hui Chu | - |
dc.contributor.googleauthor | Joo Weon Lim | - |
dc.contributor.googleauthor | Dong Goo Kim | - |
dc.contributor.googleauthor | Eung-Seok Lee | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.identifier.doi | 10.3109/00365521.2010.525255 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.contributor.localId | A00396 | - |
dc.contributor.localId | A04232 | - |
dc.relation.journalcode | J02632 | - |
dc.identifier.eissn | 1502-7708 | - |
dc.identifier.url | http://informahealthcare.com/doi/abs/10.3109/00365521.2010.525255 | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Bcl-2 | - |
dc.subject.keyword | Helicobacter pylori | - |
dc.subject.keyword | NF-κB | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.alternativeName | Kim, Dong Goo | - |
dc.contributor.alternativeName | Choo, Sang Hee | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Dong Goo | - |
dc.contributor.affiliatedAuthor | Choo, Sang Hee | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 46 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 148 | - |
dc.citation.endPage | 155 | - |
dc.identifier.bibliographicCitation | SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, Vol.46(2) : 148-155, 2011 | - |
dc.identifier.rimsid | 28621 | - |
dc.type.rims | ART | - |
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