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Altered cellular kinetics in growth plate according to alterations in weight bearing

 Hoon Park  ;  Sun Young Kong  ;  Hyun Woo Kim  ;  Ick Hwan Yang 
 YONSEI MEDICAL JOURNAL, Vol.53(3) : 618-624, 2012 
Journal Title
Issue Date
Animals ; Apoptosis/physiology ; Cell Proliferation ; Chondrocytes/cytology ; Growth Plate/cytology* ; In Situ Nick-End Labeling ; Kinetics ; Rats ; Rats, Sprague-Dawley ; Weight-Bearing/physiology*
Cellular kinetics ; growth plate ; changes in weight bearing
PURPOSE: To examine the effects of change in weight bearing on the growth plate metabolism, a simulated animal model of weightlessness was introduced and the chondrocytes' cellular kinetics was evaluated. MATERIALS AND METHODS: Unloading condition on the hind-limb of Sprague-Dawley rats was created by fixing a tail and lifting the hind-limb. Six rats aged 6 weeks old were assigned to each group of unloading, reloading, and control groups of unloading or reloading. Unloading was maintained for three weeks, and then reloading was applied for another one week thereafter. Histomorphometry for the assessment of vertical length of the growth plate, 5-bromo-2'-deoxyuridin immunohistochemistry for cellular kinetics, and biotin nick end labeling transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) assay for chondrocytes apoptosis in the growth plate were performed. RESULTS: The vertical length of the growth plate and the proliferative potential of chondrocytes were decreased in the unloading group compared to those of control groups. Inter-group differences were more significant in the proliferative and hypertrophic zones. Reloading increased the length of growth plate and proliferative potential of chondrocytes. However, apoptotic changes in the growth plate were not affected by the alterations of weight bearing. CONCLUSION: Alterations in the weight bearing induced changes in the chondrocytic proliferative potential of the growth plate, however, had no effects on the apoptosis. This may explain why non-weight bearing in various clinical situations hampers normal longitudinal bone growth. Further studies on the factors for reversibility of chondrocytic proliferation upon variable mechanical stresses are needed.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kong, Sun Young(공선영)
Kim, Hyun Woo(김현우) ORCID logo https://orcid.org/0000-0001-8576-1877
Park, Hoon(박훈) ORCID logo https://orcid.org/0000-0002-8063-3742
Yang, Ick Hwan(양익환)
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