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Altered cellular kinetics in the growth plate of the femoral head of spontaneously hypertensive rats

 Hoon Park  ;  Sun Young Kong  ;  Hyun Woo Kim 
 YONSEI MEDICAL JOURNAL, Vol.53(3) : 625-633, 2012 
Journal Title
Issue Date
Animals ; Apoptosis ; Femur Head/metabolism ; Femur Head/pathology* ; Femur Head Necrosis/metabolism ; Femur Head Necrosis/pathology ; Growth Plate/cytology* ; Growth Plate/metabolism ; Osteogenesis/physiology ; Rats ; Rats, Inbred SHR ; Rats, Sprague-Dawley ; Vascular Endothelial Growth Factor A/metabolism
Spontaneous hypertensive rats ; growth plate ; avascular necrosis ; apoptosis ; vascular endothelial growth factor
PURPOSE: Pathologic changes in the growth plate remain unknown in Legg-Calvé-Perthes (LCP) disease. Spontaneously hypertensive rats have proven to be a good model for studying LCP disease. This study investigated the histopathologic changes and the expression of vascular endothelial growth factor in the growth plate of spontaneously hypertensive rats (SHR). MATERIALS AND METHODS: Sixty SHR rats were divided into two groups: those showing osteonecrosis (SHR+n group: 32), and those showing normal ossification (SHR-n group: 28). Thirty Wister Kyoto rats served as a control. For histomorphological measurement, the length of each zone of the growth plate was measured. Cell kinetics was measured by 5-bromo-2'-deoxyuridin (BrdU) immunohistochemistry and transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) assays. Vascular endothelial growth factor (VEGF) immunohistochemistry was used to identify of expression of VEGF. RESULTS: The lengths of growth plates of the SHR+n group were significantly shorter in the initial growth period than those of the other groups. The lowest proliferative rate and the highest apoptosis rate were observed in the SHR+n group at the initial growth period. The expression of VEGF in the growth plate of the SHR group was lower than the control group, and it was lower in the SHR+n group than in the SHR-n group. CONCLUSION: The growth plate of the SHR+n group was found to be affected by disease process of ischemic necrosis of the femoral head, and this might explain the relative overgrowth of the greater trochanter in the later stages of LCP disease
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kong, Sun Young(공선영)
Kim, Hyun Woo(김현우) ORCID logo https://orcid.org/0000-0001-8576-1877
Park, Hoon(박훈) ORCID logo https://orcid.org/0000-0002-8063-3742
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