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Cellular function of RhoGDI-α mediates the cycling of Rac1 and the regulation of pancreatic beta cell death

Authors
 Y. Cho  ;  K.H. Huh  ;  Y.-J. Park  ;  J.H. Do  ;  D.J. Joo  ;  M.S. Kim  ;  Y.S. Kim 
Citation
 Transplantation Proceedings, Vol.44(4) : 1083-1085, 2012 
Journal Title
 Transplantation Proceedings 
ISSN
 0041-1345 
Issue Date
2012
Abstract
Mycophenolic acid (MPA) is an immunosuppressive agent that is widely used in clinical therapy, including pancreas and islet transplantation. Previously, we showed that MPA induces significant apoptosis of insulin-secreting cells by downregulating RhoGDI-α and increasing JNK expression. In this study, we investigated Rac1 directly associated with RhoGDI-α during MPA-induced apoptosis in INS-1E cells (an insulin-secreting cell line). Cells were treated with MPA for 24 and 36 hours. Immunoprecipitation was used to examine physical interactions between RhoGDI-α and Rac1. Activation and immunoprecipitation assays showed expressions of Rac1 and RhoGDI-α to be directly correlated. Rac1 binding to RhoGDI-α decreased after MPA treatment, and Rac1 was induced and subsequently activated by MPA. We concluded that this novel RhoGDI-α/Rac1/JNK pathway induced apoptosis of transplanted islet cells after MPA treatment.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91976
DOI
10.1016/j.transproceed.2012.03.021
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
Yonsei Authors
김명수(Kim, Myoung Soo) ; 김유선(Kim, Yu Seun) ; 도지혜(Do, Ji Hye) ; 박윤종(Park, Yun Jong) ; 조유리(Cho, Yu Ri) ; 주동진(Joo, Dong Jin) ; 허규하(Huh, Kyu Ha)
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Full Text
http://www.sciencedirect.com/science/article/pii/S0041134512002412
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