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Microencapsulation of pancreatic islets with canine ear cartilage for immunoisolation

Authors
 J.I. Lee  ;  H.W. Kim  ;  J.Y. Kim  ;  S.J. Bae  ;  D.J. Joo  ;  K.H. Huh  ;  Y.H. Fang  ;  J.H. Jeong  ;  M.S. Kim  ;  Y.S. Kim 
Citation
 Transplantation Proceedings, Vol.44(4) : 1091-1094, 2012 
Journal Title
 Transplantation Proceedings 
ISSN
 0041-1345 
Issue Date
2012
Abstract
Improving human islet transplantation is often limited by the shortage of donors and the side effects of immunosuppressive agents. If immunoisolation is properly used, it can overcome these obstacles. Because artificial materials are adopted in this technique, however, there are still multiple issues with biocompatibility and foreign body reactions. We developed a chondrocyte microencapsulated immunoisolated islet (CMI-islet) that allows living cells to act as the immunoisolating material. To manufacture CMI-islets for xenotransplantation, isolated rat pancreatic islets were placed on low cell-binding culture dishes. Subsequently, expanded canine auricular cartiage primary cells were seeded on these dishes at a high density and maintained in a suspended state via a shaking culture system. Morphological evaluations showed good islet viability and a clear progression of the islet- encapsulation events. When the cells were challenged with glucose, they were able to secrete sufficient insulin according to glucose concentrations. The CMI-islets responded better to the glucose challenge than did nude pancreatic islets and created better glucose-insulin feedback regulation. Moreover, insulin secretion into the culture medium was confirmed over a period of 100 days, showing the survival and secretory capacity of the CMI-islet cells. By microencapsulating pancreatic islets with recipient ear cartilage cells, long-term insulin secretion can be maintained and the response to glucose challenges improved. This new immunodelusion technology differs from other immunoisolation techniques in that the donor tissue is enclosed with the recipient's tissue, thus allowing the transplanted cells to be recognized as recipient cells. This microencapsulation method may lead to developing viable xenotransplantation techniques that do not use immunosuppressive drugs.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91969
DOI
10.1016/j.transproceed.2012.02.015
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실)
Yonsei Authors
김명수(Kim, Myoung Soo) ; 김유선(Kim, Yu Seun) ; 김준예(Kim, Joon Ye) ; 방우휘(Fang, Yu Hui) ; 정진호(Jeong, Jin Ho) ; 주동진(Joo, Dong Jin) ; 허규하(Huh, Kyu Ha)
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Full Text
http://www.sciencedirect.com/science/article/pii/S0041134512002035
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