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Evaluating the genomic and sequence integrity of human ES cell lines; comparison to normal genomes

 Walter D. Funk  ;  Ivan Labat  ;  Janani Sampathkumar  ;  Pierre-Antoine Gourraud  ;  Jorge R. Oksenberg  ;  Elen Rosler  ;  Daniel Steiger  ;  Nadia Sheibani  ;  Stacy Caillier  ;  Birgit Stache-Crain  ;  Julie A. Johnson  ;  Lorraine Meisner  ;  Markus D. Lacher  ;  Karen B. Chapman  ;  Myung Jin Park  ;  Kyoung-Jin Shin  ;  Rade Drmanac  ;  Michael D. West 
 Stem Cell Research, Vol.8(2) : 154-164, 2012 
Journal Title
 Stem Cell Research 
Issue Date
Copy number variation (CNV) is a common chromosomal alteration that can occur during in vitro cultivation of human cells and can be accompanied by the accumulation of mutations in coding region sequences. We describe here a systematic application of current molecular technologies to provide a detailed understanding of genomic and sequence profiles of human embryonic stem cell (hESC) lines that were derived under GMP-compliant conditions. We first examined the overall chromosomal integrity using cytogenetic techniques to determine chromosome count, and to detect the presence of cytogenetically aberrant cells in the culture (mosaicism). Assays of copy number variation, using both microarray and sequence-based analyses, provide a detailed view genomic variation in these lines and shows that in early passage cultures of these lines, the size range and distribution of CNVs are entirely consistent with those seen in the genomes of normal individuals. Similarly, genome sequencing shows variation within these lines that is completely within the range seen in normal genomes. Important gene classes, such as tumor suppressors and genetic disease genes, do not display overtly disruptive mutations that could affect the overall safety of cell-based therapeutics. Complete sequence also allows the analysis of important transplantation antigens, such as ABO and HLA types. The combined application of cytogenetic and molecular technologies provides a detailed understanding of genomic and sequence profiles of GMP produced ES lines for potential use as therapeutic agents.
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1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과)
Yonsei Authors
박명진(Park, Myung Jin)
신경진(Shin, Kyoung Jin) ORCID logo https://orcid.org/0000-0002-1059-9665
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