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Triamcinolone decreases bupivacaine toxicity to intervertebral disc cell in vitro

Authors
 Ju-Hyung Moon  ;  Sung-Uk Kuh,  ;  Hyo-Suk Park  ;  Kyung-Hyun Kim  ;  Jeong-Yoon Park  ;  Dong-Kyu Chin  ;  Keun-Su Kim  ;  Yong-Eun Cho 
Citation
 Spine Journal, Vol.12(8) : 665-673, 2012 
Journal Title
 Spine Journal 
ISSN
 1529-9430 
Issue Date
2012
Abstract
BACKGROUND CONTEXT: Local anesthetics combined with corticosteroids are commonly used for management of back pain in interventional spinal procedures. Several recent studies suggest cytotoxicity of bupivacaine, whereas others report protective and cytotoxic effects of corticosteroids on chondrocytes and intervertebral disc cells. Considering the frequent use of these agents in spinal interventions, it is meaningful to know how they affect intervertebral disc cells. PURPOSE: This study was conducted to assess the effects of bupivacaine and triamcinolone, both alone and in combination, on viability of intervertebral disc cells in vitro. STUDY DESIGN: Controlled laboratory study. METHODS: Nucleus pulposus cells were isolated from human disc specimens from patients undergoing surgery because of disc herniation or degenerative disc disease. They were grown in three-dimensional alginate beads for 1 week to maintain their differentiated phenotypes and to allow for matrix formation before analysis. After 1 week of culture, the cells were exposed to bupivacaine (0.1%, 0.25%, 0.5%, and 1%) or bupivacaine (0.1%, 0.25%, 0.5%, and 1%) with 1 mg of triamcinolone for 1, 3, or 6 hours. Cell viability was measured using trypan blue exclusion assay and flow cytometry. Live cell/dead cell fluorescent imaging was assessed using confocal microscopy. RESULTS: Trypan blue exclusion assays demonstrated dose- and time-dependent cytotoxic effects of bupivacaine on human nucleus pulposus cells. Similar but reduced cytotoxicity was observed after exposure to the combination of bupivacaine and 1 mg of triamcinolone. Flow cytometry showed a dose-dependent cytotoxic effect of bupivacaine on nucleus pulposus cells after 3 hours of exposure. The reduced cytotoxicity of bupivacaine combined with 1 mg of triamcinolone was also confirmed in flow cytometry. Confocal images showed that the increase in dead cells correlated with the concentration of bupivacaine. Nevertheless, fewer cells died after exposure to several different concentrations of bupivacaine combined with 1 mg of triamcinolone than did after exposure to bupivacaine alone. CONCLUSIONS: The combination of bupivacaine and triamcinolone induced dose- and time-dependent cytotoxicity on human intervertebral disc cells in vitro, but the cytotoxicity was much weaker than that of bupivacaine alone. This study shows a potential protective influence of triamcinolone on intervertebral disc cells.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91864
DOI
10.1016/j.spinee.2012.06.009
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실)
Yonsei Authors
구성욱(Kuh, Sung Uk) ; 김경현(Kim, Kyung Hyun) ; 김근수(Kim, Keun Su) ; 박정윤(Park, Jeong Yoon) ; 박형천(Park, Hyeong Cheon) ; 조용은(Cho, Yong Eun) ; 진동규(Chin, Dong Kyu)
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Full Text
http://www.sciencedirect.com/science/article/pii/S1529943012004330
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