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Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells

Authors
 HYUNSOO SHIN  ;  YEO SONG LEE  ;  YONG CHAN LEE 
Citation
 ONCOLOGY REPORTS, Vol.27(4) : 1111-1115, 2012 
Journal Title
 ONCOLOGY REPORTS 
ISSN
 1021-335X 
Issue Date
2012
MeSH
Antineoplastic Agents/pharmacology* ; Apoptosis/drug effects* ; Apoptosis Regulatory Proteins/metabolism* ; Butyrates/pharmacology* ; Calcium-Calmodulin-Dependent Protein Kinases/metabolism* ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Cyclin-Dependent Kinase Inhibitor p21 ; Death-Associated Protein Kinases ; Fluorescent Antibody Technique ; Focal Adhesion Kinase 1/metabolism ; Histone Deacetylase Inhibitors/pharmacology* ; Humans ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Stomach Neoplasms/enzymology* ; Stomach Neoplasms/pathology ; Time Factors ; Tumor Suppressor Protein p53/metabolism ; Up-Regulation
Keywords
Antineoplastic Agents/pharmacology* ; Apoptosis/drug effects* ; Apoptosis Regulatory Proteins/metabolism* ; Butyrates/pharmacology* ; Calcium-Calmodulin-Dependent Protein Kinases/metabolism* ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Cyclin-Dependent Kinase Inhibitor p21 ; Death-Associated Protein Kinases ; Fluorescent Antibody Technique ; Focal Adhesion Kinase 1/metabolism ; Histone Deacetylase Inhibitors/pharmacology* ; Humans ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Stomach Neoplasms/enzymology* ; Stomach Neoplasms/pathology ; Time Factors ; Tumor Suppressor Protein p53/metabolism ; Up-Regulation
Abstract
Epigenetic mechanisms of histone acetylation/deacetylation play an important role in the regulation of gene expression associated with the cell cycle and apoptosis. Recently, sodium butyrate, a histone deacetylase (HDAC) inhibitor, has been shown to exhibit anticancer effects via differentiation and apoptosis of cancer cells. Sodium butyrate may be a potential anticancer chemotherapeutic drug; however, the precise mechanism underlying the anticancer effects of sodium butyrate has not been clearly elucidated. In the present study, we investigated the role of death-associated protein kinase (DAPK) on the apoptosis of human gastric cancer cells induced by sodium butyrate. We observed that sodium butyrate induced apoptosis in human gastric cancer cells. Treatment with the HDAC inhibitor sodium butyrate increased the expression of caspase-3 and DAPK1/2 genes but decreased the expression of Bcl-2 in human gastric cancer cells. The expression of DAPK3, p53 and p21 were not altered by sodium butyrate treatment. Analysis of the general expression patterns revealed that sodium butyrate increased the expression of DAPK1/2 but decreased the expression of FAK and induced changes in the proliferation of apoptosis-related genes in human gastric cancer cells. These data suggest that DAPK expression prompts apoptosis by reducing the FAK protein level in sodium butyrate-induced apoptosis of human gastric cancer cells.
Files in This Item:
T201201497.pdf Download
DOI
22160140
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Lee, Yeo Song(이여송)
Lee, Yong Chan(이용찬) ORCID logo https://orcid.org/0000-0001-8800-6906
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91591
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