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Immediately transcripted genes in various hepatic ischemia models

Authors
 Kang Kook Choi  ;  Jin A Cho  ;  Se Hoon Kim  ;  Sang Woo Lee  ;  Seon Ok Min  ;  Kyung Sik Kim 
Citation
 Journal of the Korean Surgical Society, Vol.83(5) : 298-306, 2012 
Journal Title
 Journal of the Korean Surgical Society 
ISSN
 2233-7903 
Issue Date
2012
Keywords
Apoptosis ; Ischemic preconditioning ; Microarray analysis ; Necrosis ; Reperfusion injury
Abstract
PURPOSE: To elucidate the characteristic gene transcription profiles among various hepatic ischemia conditions, immediately transcribed genes and the degree of ischemic injury were compared among total ischemia (TI), intermittent clamping (IC), and ischemic preconditioning (IPC). METHODS: Sprague-Dawley rats were equally divided into control (C, sham-operated), TI (ischemia for 90 minutes), IC (ischemia for 15 minutes and reperfusion for 5 minutes, repeated six times), and IPC (ischemia for 15 minutes, reperfusion for 5 minutes, and ischemia again for 90 minutes) groups. A cDNA microarray analysis was performed using hepatic tissues obtained by partial hepatectomy after occluding hepatic inflow. RESULTS: THE CDNA MICROARRAY REVEALED THE FOLLOWING: interleukin (IL)-1β expression was 2-fold greater in the TI group than in the C group. In the IC group, IL-1α/β expression increased by 2.5-fold, and Na+/K+ ATPase β1 expression decreased by 2.4-fold. In the IPC group, interferon regulatory factor-1, osteoprotegerin, and retinoblastoma-1 expression increased by approximately 2-fold compared to that in the C group, but the expression of Na+/K+ ATPase β1 decreased 3-fold. CONCLUSION: The current findings revealed characteristic gene expression profiles under various ischemic conditions. However, additional studies are needed to clarify the mechanism of protection against IPC.
Files in This Item:
T201205228.pdf Download
DOI
23166889
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sik(김경식) ORCID logo https://orcid.org/0000-0001-9498-284X
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Min, Seon Ok(민선옥)
Lee, Sang Woo(이상우)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91161
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