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Cited 14 times in

Combination of radiotherapy and adenovirus-mediated p53 gene therapy for MDM2-overexpressing hepatocellular carcinoma

DC FieldValueLanguage
dc.contributor.author성진실-
dc.contributor.author윤채옥-
dc.contributor.author최일규-
dc.contributor.author금웅섭-
dc.contributor.author김원우-
dc.contributor.author김지성-
dc.contributor.author김현기-
dc.contributor.author박수연-
dc.date.accessioned2014-12-19T17:15:44Z-
dc.date.available2014-12-19T17:15:44Z-
dc.date.issued2012-
dc.identifier.issn0449-3060-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91080-
dc.description.abstractThe p53 gene plays a determinant role in radiation-induced cell death and its protein product is negatively regulated by MDM2. We investigated whether adenovirus-mediated modified p53 gene transfer, which blocks p53-MDM2 binding, is effective for radiation-induced cell death in hepatocellular carcinoma (HCC) at different MDM2 cellular levels. Human hepatocellular carcinoma cell lines expressing MDM2 at low levels (Huh7) and high levels (SK-Hep1) were used. Ad-p53 and Ad-p53vp are replication-deficient adenoviral vectors containing human wild-type or modified p53, respectively. The anti-tumor effect was highest for Ad-p53 + radiotherapy (RT) in the low-level MDM2 cells, whereas this effect was highest for Ad-p53vp + RT in the MDM2-overexpressing cells. In Huh-7 cells, Ad-p53 + RT decreased cell viability (32%) in vitro and inhibited tumor growth (enhancement factor, 1.86) in vivo. Additionally, p21 expression and apoptosis were increased. In contrast, in SK-Hep1 cells, Ad-p53vp + RT showed decreased cell viability (51%) in vitro and inhibition of tumor growth (enhancement factor, 3.07) in vivo. Caspase-3 expression and apoptosis were also increased. Adenovirus-expressing modified p53, which blocks p53-MDM2 binding, was effective in killing tumor cells overexpressing MDM2. Furthermore, the combination strategy for disruption of the p53-MDM2 interaction with RT demonstrated enhanced anti-tumor effects both in vitro and in vivo.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJournal of Radiation Research-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleCombination of radiotherapy and adenovirus-mediated p53 gene therapy for MDM2-overexpressing hepatocellular carcinoma-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pathology (병리학)-
dc.contributor.googleauthorWoong Sub KOOM-
dc.contributor.googleauthorSoo-Yeon PARK-
dc.contributor.googleauthorWonwoo KIM-
dc.contributor.googleauthorMinjung KIM-
dc.contributor.googleauthorJi-Seong KIM-
dc.contributor.googleauthorHyunki KIM-
dc.contributor.googleauthorIl-Kyu CHOI-
dc.contributor.googleauthorChae-Ok YUN-
dc.contributor.googleauthorJinsil SEONG-
dc.identifier.doi10.1269/jrr.11110-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01956-
dc.contributor.localIdA02614-
dc.contributor.localIdA04168-
dc.contributor.localIdA00273-
dc.contributor.localIdA00768-
dc.contributor.localIdA00967-
dc.contributor.localIdA01108-
dc.contributor.localIdA01534-
dc.relation.journalcodeJ01727-
dc.contributor.alternativeNameSeong, Jin Sil-
dc.contributor.alternativeNameYun, Chae Ok-
dc.contributor.alternativeNameChoi, Il Kyu-
dc.contributor.alternativeNameKoom, Woong Sub-
dc.contributor.alternativeNameKim, Won Woo-
dc.contributor.alternativeNameKim, Ji Seong-
dc.contributor.alternativeNameKim, Hyun Ki-
dc.contributor.alternativeNamePark, Soo Yeon-
dc.contributor.affiliatedAuthorSeong, Jin Sil-
dc.contributor.affiliatedAuthorYun, Chae Ok-
dc.contributor.affiliatedAuthorChoi, Il Kyu-
dc.contributor.affiliatedAuthorKoom, Woong Sub-
dc.contributor.affiliatedAuthorKim, Won Woo-
dc.contributor.affiliatedAuthorKim, Ji Seong-
dc.contributor.affiliatedAuthorKim, Hyun Ki-
dc.contributor.affiliatedAuthorPark, Soo Yeon-
dc.citation.volume53-
dc.citation.number2-
dc.citation.startPage202-
dc.citation.endPage210-
dc.identifier.bibliographicCitationJournal of Radiation Research, Vol.53(2) : 202-210, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
5. Research Institutes (연구소) > Institute for Cancer Research (암연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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