Cited 25 times in
Combination of radiotherapy and adenovirus-mediated p53 gene therapy for MDM2-overexpressing hepatocellular carcinoma
DC Field | Value | Language |
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dc.contributor.author | 성진실 | - |
dc.contributor.author | 윤채옥 | - |
dc.contributor.author | 최일규 | - |
dc.contributor.author | 금웅섭 | - |
dc.contributor.author | 김원우 | - |
dc.contributor.author | 김지성 | - |
dc.contributor.author | 김현기 | - |
dc.contributor.author | 박수연 | - |
dc.date.accessioned | 2014-12-19T17:15:44Z | - |
dc.date.available | 2014-12-19T17:15:44Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0449-3060 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91080 | - |
dc.description.abstract | The p53 gene plays a determinant role in radiation-induced cell death and its protein product is negatively regulated by MDM2. We investigated whether adenovirus-mediated modified p53 gene transfer, which blocks p53-MDM2 binding, is effective for radiation-induced cell death in hepatocellular carcinoma (HCC) at different MDM2 cellular levels. Human hepatocellular carcinoma cell lines expressing MDM2 at low levels (Huh7) and high levels (SK-Hep1) were used. Ad-p53 and Ad-p53vp are replication-deficient adenoviral vectors containing human wild-type or modified p53, respectively. The anti-tumor effect was highest for Ad-p53 + radiotherapy (RT) in the low-level MDM2 cells, whereas this effect was highest for Ad-p53vp + RT in the MDM2-overexpressing cells. In Huh-7 cells, Ad-p53 + RT decreased cell viability (32%) in vitro and inhibited tumor growth (enhancement factor, 1.86) in vivo. Additionally, p21 expression and apoptosis were increased. In contrast, in SK-Hep1 cells, Ad-p53vp + RT showed decreased cell viability (51%) in vitro and inhibition of tumor growth (enhancement factor, 3.07) in vivo. Caspase-3 expression and apoptosis were also increased. Adenovirus-expressing modified p53, which blocks p53-MDM2 binding, was effective in killing tumor cells overexpressing MDM2. Furthermore, the combination strategy for disruption of the p53-MDM2 interaction with RT demonstrated enhanced anti-tumor effects both in vitro and in vivo. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF RADIATION RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Combination of radiotherapy and adenovirus-mediated p53 gene therapy for MDM2-overexpressing hepatocellular carcinoma | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | Woong Sub KOOM | - |
dc.contributor.googleauthor | Soo-Yeon PARK | - |
dc.contributor.googleauthor | Wonwoo KIM | - |
dc.contributor.googleauthor | Minjung KIM | - |
dc.contributor.googleauthor | Ji-Seong KIM | - |
dc.contributor.googleauthor | Hyunki KIM | - |
dc.contributor.googleauthor | Il-Kyu CHOI | - |
dc.contributor.googleauthor | Chae-Ok YUN | - |
dc.contributor.googleauthor | Jinsil SEONG | - |
dc.identifier.doi | 10.1269/jrr.11110 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01956 | - |
dc.contributor.localId | A02614 | - |
dc.contributor.localId | A04168 | - |
dc.contributor.localId | A00273 | - |
dc.contributor.localId | A00768 | - |
dc.contributor.localId | A00967 | - |
dc.contributor.localId | A01108 | - |
dc.contributor.localId | A01534 | - |
dc.relation.journalcode | J01727 | - |
dc.identifier.eissn | 1349-9157 | - |
dc.contributor.alternativeName | Seong, Jin Sil | - |
dc.contributor.alternativeName | Yun, Chae Ok | - |
dc.contributor.alternativeName | Choi, Il Kyu | - |
dc.contributor.alternativeName | Koom, Woong Sub | - |
dc.contributor.alternativeName | Kim, Won Woo | - |
dc.contributor.alternativeName | Kim, Ji Seong | - |
dc.contributor.alternativeName | Kim, Hyun Ki | - |
dc.contributor.alternativeName | Park, Soo Yeon | - |
dc.contributor.affiliatedAuthor | Seong, Jin Sil | - |
dc.contributor.affiliatedAuthor | Yun, Chae Ok | - |
dc.contributor.affiliatedAuthor | Choi, Il Kyu | - |
dc.contributor.affiliatedAuthor | Koom, Woong Sub | - |
dc.contributor.affiliatedAuthor | Kim, Won Woo | - |
dc.contributor.affiliatedAuthor | Kim, Ji Seong | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Ki | - |
dc.contributor.affiliatedAuthor | Park, Soo Yeon | - |
dc.citation.volume | 53 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 202 | - |
dc.citation.endPage | 210 | - |
dc.identifier.bibliographicCitation | JOURNAL OF RADIATION RESEARCH, Vol.53(2) : 202-210, 2012 | - |
dc.identifier.rimsid | 33319 | - |
dc.type.rims | ART | - |
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