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In-vivo and in-vitro biocompatibility evaluations of silver nanoparticles with antimicrobial activity

Authors
 Han, Dong-Wook  ;  Woo, Yeon I  ;  Lee, Mi Hee  ;  Lee, Jong Ho  ;  Lee, Jaebeom  ;  Park, Jong-Chul 
Citation
 JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, Vol.12(7) : 5205-5209, 2012 
Journal Title
 JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 
ISSN
 1533-4880 
Issue Date
2012
MeSH
Animals ; Anti-Bacterial Agents/adverse effects ; Anti-Bacterial Agents/pharmacology* ; Bacterial Physiological Phenomena/drug effects* ; Biocompatible Materials/adverse effects ; Biocompatible Materials/pharmacology ; CHO Cells ; Cricetinae ; Cricetulus ; Lethal Dose 50 ; Materials Testing ; Metal Nanoparticles/administration & dosage* ; Metal Nanoparticles/adverse effects ; Mice
Keywords
Animals ; Anti-Bacterial Agents/adverse effects ; Anti-Bacterial Agents/pharmacology* ; Bacterial Physiological Phenomena/drug effects* ; Biocompatible Materials/adverse effects ; Biocompatible Materials/pharmacology ; CHO Cells ; Cricetinae ; Cricetulus ; Lethal Dose 50 ; Materials Testing ; Metal Nanoparticles/administration & dosage* ; Metal Nanoparticles/adverse effects ; Mice
Abstract
In this study, the biocompatibility and antimicrobial activity of silver nanoparticles (Ag NPs) were evaluated in vitro and in vivo. The cytotoxicity of Ag NPs (average diameter: 2-5 nm) against CHO-K1 cells was determined via WST-8 assay, and their genotoxicity was examined via Salmonella typhimurium reverse mutation assay (Ames test). The acute toxicity and intracutaneous reactivity of Ag NPs were evaluated using animal models of mice and rabbits, respectively. The antibacterial effects of Ag NPs on the Gram (-) bacterial strains of Escherichia coli ATCC 8739 and Pseudomonas aeruginosa ATCC 9027 and on the Gram (+) bacterial strains of Staphylococcus aureus ATCC 6538p and Bacillus subtilius ATCC 6633 were determined by measuring the minimum inhibitory concentrations. The Ag NPs were highly cytotoxic to the L-929 cells at over 2 ppm but were non-cytotoxic at lower than 1 ppm. Moreover, the Ag NPs at 1 ppm or lower did not show genotoxicity, acute toxicity and intracutaneous reactivity. It was also found that the Ag NPs exerted effective antimicrobial activities on both the Gram (-) and (+) bacterial strains within the range from 0.06 to 0.98 ppm for 50% MIC.
Full Text
http://www.ingentaconnect.com/content/asp/jnn/2012/00000012/00000007/art00015
DOI
22966546
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Woo, Yeon I.(우연이)
Lee, Mi Hee(이미희) ORCID logo https://orcid.org/0000-0002-9630-7044
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90980
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