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Immortalized gingival fibroblasts as a cytotoxicity test model for dental materials

 Rasika P. Illeperuma  ;  Young J. Park  ;  Jin M. Kim  ;  Jung Y. Bae  ;  Zhong M. Che  ;  Hwa K. Son  ;  Mi R. Han  ;  Kwang M. Kim  ;  Jin Kim 
 Journal of Materials Science-Materials in Medicine, Vol.23(3) : 753-762, 2012 
Journal Title
 Journal of Materials Science-Materials in Medicine 
Issue Date
Cell Cycle ; Cell Line, Transformed ; Cell Proliferation ; Dental Materials* ; Fibroblasts/cytology ; Flow Cytometry ; Gingiva/cytology* ; Humans
L929 Cell ; Cytotoxicity Testing ; Parental Cell Line ; Replicative Senescence ; Dental Material
In vitro cytotoxicity test is an initial step to identify the harmful effects of new dental materials. Aim of this study was to develop a stable human cell line derived from normal gingival fibroblasts (hNOF) and to assess its feasibility in in vitro cytotoxicity testing. Immortalized human gingival fibroblasts (hTERT-hNOF) were successfully established with human telomerase reverse transcriptase gene transfection, preserving its phenotypical characteristics, replicative potential and biological properties. Utilizing standard cytotoxicity test modeling and dental materials, hTERT-hNOF were evaluated for their feasibility in cytotoxicity testing, compared with hNOF and L929 cells. Similar pattern of cytotoxic response was observed among hNOF, hTERT-hNOF and L929 cells. Cytotoxicity response of hTERT-hNOF was significantly similar to hNOF, moreover hTERT-hNOF and hNOF were found to be more sensitive towards the tested dental materials compared to L929 cells. This study suggested that hTERT-hNOF is an effective cytotoxic test model for dental materials.
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Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Kim, Jin(김진)
Kim, Jin Mi(김진미)
Park, Young Jin(박영진)
Bae, Jung Yoon(배정윤) ORCID logo https://orcid.org/0000-0001-8342-6987
Son, Hwa Kyung(손화경)
Han, Mi Ra(한미라)
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