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Immortalized gingival fibroblasts as a cytotoxicity test model for dental materials

 Rasika P. Illeperuma  ;  Young J. Park  ;  Jin M. Kim  ;  Jung Y. Bae  ;  Zhong M. Che  ;  Hwa K. Son  ;  Mi R. Han  ;  Kwang M. Kim  ;  Jin Kim 
 Journal of Materials Science-Materials in Medicine, Vol.23(3) : 753-762, 2012 
Journal Title
 Journal of Materials Science-Materials in Medicine 
Issue Date
In vitro cytotoxicity test is an initial step to identify the harmful effects of new dental materials. Aim of this study was to develop a stable human cell line derived from normal gingival fibroblasts (hNOF) and to assess its feasibility in in vitro cytotoxicity testing. Immortalized human gingival fibroblasts (hTERT-hNOF) were successfully established with human telomerase reverse transcriptase gene transfection, preserving its phenotypical characteristics, replicative potential and biological properties. Utilizing standard cytotoxicity test modeling and dental materials, hTERT-hNOF were evaluated for their feasibility in cytotoxicity testing, compared with hNOF and L929 cells. Similar pattern of cytotoxic response was observed among hNOF, hTERT-hNOF and L929 cells. Cytotoxicity response of hTERT-hNOF was significantly similar to hNOF, moreover hTERT-hNOF and hNOF were found to be more sensitive towards the tested dental materials compared to L929 cells. This study suggested that hTERT-hNOF is an effective cytotoxic test model for dental materials.
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5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
김광만(Kim, Kwang Mahn)
김진(Kim, Jin)
김진미(Kim, Jin Mi)
박영진(Park, Young Jin)
배정윤(Bae, Jung Yoon) ORCID logo https://orcid.org/0000-0001-8342-6987
손화경(Son, Hwa Kyung)
한미라(Han, Mi Ra)
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