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Immortalized gingival fibroblasts as a cytotoxicity test model for dental materials

DC Field Value Language
dc.contributor.author한미라-
dc.contributor.author김광만-
dc.contributor.author김진-
dc.contributor.author김진미-
dc.contributor.author박영진-
dc.contributor.author배정윤-
dc.contributor.author손화경-
dc.contributor.author배정윤-
dc.date.accessioned2014-12-19T17:11:47Z-
dc.date.available2014-12-19T17:11:47Z-
dc.date.issued2012-
dc.identifier.issn0957-4530-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90955-
dc.description.abstractIn vitro cytotoxicity test is an initial step to identify the harmful effects of new dental materials. Aim of this study was to develop a stable human cell line derived from normal gingival fibroblasts (hNOF) and to assess its feasibility in in vitro cytotoxicity testing. Immortalized human gingival fibroblasts (hTERT-hNOF) were successfully established with human telomerase reverse transcriptase gene transfection, preserving its phenotypical characteristics, replicative potential and biological properties. Utilizing standard cytotoxicity test modeling and dental materials, hTERT-hNOF were evaluated for their feasibility in cytotoxicity testing, compared with hNOF and L929 cells. Similar pattern of cytotoxic response was observed among hNOF, hTERT-hNOF and L929 cells. Cytotoxicity response of hTERT-hNOF was significantly similar to hNOF, moreover hTERT-hNOF and hNOF were found to be more sensitive towards the tested dental materials compared to L929 cells. This study suggested that hTERT-hNOF is an effective cytotoxic test model for dental materials.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHCell Cycle-
dc.subject.MESHCell Line, Transformed-
dc.subject.MESHCell Proliferation-
dc.subject.MESHDental Materials*-
dc.subject.MESHFibroblasts/cytology-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHGingiva/cytology*-
dc.subject.MESHHumans-
dc.titleImmortalized gingival fibroblasts as a cytotoxicity test model for dental materials-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentOral Cancer Research Institute (구강종양연구소)-
dc.contributor.googleauthorRasika P. Illeperuma-
dc.contributor.googleauthorYoung J. Park-
dc.contributor.googleauthorJin M. Kim-
dc.contributor.googleauthorJung Y. Bae-
dc.contributor.googleauthorZhong M. Che-
dc.contributor.googleauthorHwa K. Son-
dc.contributor.googleauthorMi R. Han-
dc.contributor.googleauthorKwang M. Kim-
dc.contributor.googleauthorJin Kim-
dc.identifier.doi22071981-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04279-
dc.contributor.localIdA00312-
dc.contributor.localIdA01015-
dc.contributor.localIdA02003-
dc.contributor.localIdA01009-
dc.contributor.localIdA01571-1-
dc.relation.journalcodeJ01576-
dc.identifier.eissn1573-4838-
dc.identifier.pmid22071981-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs10856-011-4473-6-
dc.subject.keywordL929 Cell-
dc.subject.keywordCytotoxicity Testing-
dc.subject.keywordParental Cell Line-
dc.subject.keywordReplicative Senescence-
dc.subject.keywordDental Material-
dc.contributor.alternativeNameHan, Mi Ra-
dc.contributor.alternativeNameKim, Kwang Mahn-
dc.contributor.alternativeNameKim, Jin-
dc.contributor.alternativeNameKim, Jin Mi-
dc.contributor.alternativeNamePark, Young Jin-
dc.contributor.alternativeNameBae, Jung Yoon-
dc.contributor.alternativeNameSon, Hwa Kyung-
dc.contributor.affiliatedAuthorHan, Mi Ra-
dc.contributor.affiliatedAuthorKim, Kwang Mahn-
dc.contributor.affiliatedAuthorKim, Jin Mi-
dc.contributor.affiliatedAuthorSon, Hwa Kyung-
dc.contributor.affiliatedAuthorKim, Jin-
dc.contributor.affiliatedAuthorPark, Young Jin-
dc.citation.volume23-
dc.citation.number3-
dc.citation.startPage753-
dc.citation.endPage762-
dc.identifier.bibliographicCitationJOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, Vol.23(3) : 753-762, 2012-
dc.identifier.rimsid33239-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers

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