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Detection of Mycobacterium kansasii Using DNA-DNA Detection of Mycobacterium kansasii Using DNA-DNA Hybridization with rpoB Probe

 Tae-Dong Kweon  ;  Sun-Joon Bai  ;  Chang-Shik Choi  ;  Seong-Karp Hong 
 Journal of Information and Communication Convergence Engineering, Vol.10(2) : 210-214, 2012 
Journal Title
 Journal of Information and Communication Convergence Engineering 
Issue Date
DNA ; Hybridization ; Mycobacterium kansasii
A microtiter well plate DNA hybridization method using Mycobacterium kansasii-specific rpoB DNA probe (kanp) were evaluated for the detection of M. kansasii from culture isolates. Among the 201 isolates tested by this method, 27 strains show positive results for M. kansasii, but the other 174 isolates were negative results for M. kansasii. This result was consistent with partial rpoB sequence analysis of M. kansasii and the result of biochemical tests. The negative strains by this DNA-DNA hybridization method were identified as Mycobacterium tuberculosis (159 strains), Mycobacterium avim (5 strains), Mycobacterium intracellulare (8 strains), and Mycobacterium flavescens (2 strain) by rpoB DNA sequence analysis. Due to high sensitivity and specificity of this test result, we suggest that DNA-DNA hybridization method using rpoB DNA probes of M. kansasii could be used for the rapid and convenient detection of M. kansasii.
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1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Kweon, Tae Dong(권태동) ORCID logo https://orcid.org/0000-0002-5451-1856
Bai, Sun Joon(배선준) ORCID logo https://orcid.org/0000-0001-5027-3232
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