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Detection of Mycobacterium kansasii Using DNA-DNA Detection of Mycobacterium kansasii Using DNA-DNA Hybridization with rpoB Probe
DC Field | Value | Language |
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dc.contributor.author | 권태동 | - |
dc.contributor.author | 배선준 | - |
dc.date.accessioned | 2014-12-19T17:08:35Z | - |
dc.date.available | 2014-12-19T17:08:35Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 2234-8255 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/90856 | - |
dc.description.abstract | A microtiter well plate DNA hybridization method using Mycobacterium kansasii-specific rpoB DNA probe (kanp) were evaluated for the detection of M. kansasii from culture isolates. Among the 201 isolates tested by this method, 27 strains show positive results for M. kansasii, but the other 174 isolates were negative results for M. kansasii. This result was consistent with partial rpoB sequence analysis of M. kansasii and the result of biochemical tests. The negative strains by this DNA-DNA hybridization method were identified as Mycobacterium tuberculosis (159 strains), Mycobacterium avim (5 strains), Mycobacterium intracellulare (8 strains), and Mycobacterium flavescens (2 strain) by rpoB DNA sequence analysis. Due to high sensitivity and specificity of this test result, we suggest that DNA-DNA hybridization method using rpoB DNA probes of M. kansasii could be used for the rapid and convenient detection of M. kansasii. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | Journal of Information and Communication Convergence Engineering | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Detection of Mycobacterium kansasii Using DNA-DNA Detection of Mycobacterium kansasii Using DNA-DNA Hybridization with rpoB Probe | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anesthesiology (마취통증의학) | - |
dc.contributor.googleauthor | Tae-Dong Kweon | - |
dc.contributor.googleauthor | Sun-Joon Bai | - |
dc.contributor.googleauthor | Chang-Shik Choi | - |
dc.contributor.googleauthor | Seong-Karp Hong | - |
dc.identifier.doi | 10.6109/jicce.2012.10.2.205 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00255 | - |
dc.contributor.localId | A01795 | - |
dc.relation.journalcode | J01455 | - |
dc.identifier.pmid | DNA ; Hybridization ; Mycobacterium kansasii | - |
dc.subject.keyword | DNA | - |
dc.subject.keyword | Hybridization | - |
dc.subject.keyword | Mycobacterium kansasii | - |
dc.contributor.alternativeName | Kweon, Tae Dong | - |
dc.contributor.alternativeName | Bai, Sun Joon | - |
dc.contributor.affiliatedAuthor | Kweon, Tae Dong | - |
dc.contributor.affiliatedAuthor | Bai, Sun Joon | - |
dc.citation.volume | 10 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 210 | - |
dc.citation.endPage | 214 | - |
dc.identifier.bibliographicCitation | Journal of Information and Communication Convergence Engineering, Vol.10(2) : 210-214, 2012 | - |
dc.identifier.rimsid | 34567 | - |
dc.type.rims | ART | - |
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