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Comparison of the Abbott RealTime High-Risk Human Papillomavirus (HPV), Roche Cobas HPV, and Hybrid Capture 2 assays to direct sequencing and genotyping of HPV DNA

Authors
 Yongjung Park  ;  Eunhee Lee  ;  Jonghyeon Choi  ;  Seri Jeong  ;  Hyon-Suk Kim 
Citation
 JOURNAL OF CLINICAL MICROBIOLOGY, Vol.50(7) : 2359-2365, 2012 
Journal Title
 JOURNAL OF CLINICAL MICROBIOLOGY 
ISSN
 0095-1137 
Issue Date
2012
MeSH
Cervix Uteri/virology ; DNA, Viral/chemistry ; DNA, Viral/genetics ; Female ; Genotype ; Humans ; Molecular Diagnostic Techniques/methods* ; Nucleic Acid Hybridization ; Papillomaviridae/classification* ; Papillomaviridae/genetics ; Papillomaviridae/isolation & purification* ; Papillomavirus Infections/diagnosis* ; Papillomavirus Infections/virology* ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Sequence Analysis, DNA ; Virology/methods*
Keywords
Cervix Uteri/virology ; DNA, Viral/chemistry ; DNA, Viral/genetics ; Female ; Genotype ; Humans ; Molecular Diagnostic Techniques/methods* ; Nucleic Acid Hybridization ; Papillomaviridae/classification* ; Papillomaviridae/genetics ; Papillomaviridae/isolation & purification* ; Papillomavirus Infections/diagnosis* ; Papillomavirus Infections/virology* ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Sequence Analysis, DNA ; Virology/methods*
Abstract
Infection with high-risk (HR) human papillomavirus (HPV) genotypes is an important risk factor for cervical cancers. We evaluated the clinical performances of two new real-time PCR assays for detecting HR HPVs compared to that of the Hybrid Capture 2 test (HC2). A total of 356 cervical swab specimens, which had been examined for cervical cytology, were assayed by Abbott RealTime HR and Roche Cobas HPV as well as HC2. Sensitivities and specificities of these assays were determined based on the criteria that concordant results among the three assays were regarded as true-positive or -negative and that the results of genotyping and sequencing were considered true findings when the HPV assays presented discrepant results. The overall concordance rate among the results for the three assays was 82.6%, and RealTime HR and Cobas HPV assays agreed with HC2 in 86.1% and 89.9% of cases, respectively. The two real-time PCR assays agreed with each other for 89.6% of the samples, and the concordance rate between them was equal to or greater than 98.0% for detecting HPV type 16 or 18. HC2 demonstrated a sensitivity of 96.6% with a specificity of 89.1% for detecting HR HPVs, while RealTime HR presented a sensitivity of 78.3% with a specificity of 99.2%. The sensitivity and specificity of Cobas HPV for detecting HR HPVs were 91.7% and 97.0%. The new real-time PCR assays exhibited lower sensitivities for detecting HR HPVs than that of HC2. Nevertheless, the newly introduced assays have an advantage of simultaneously identifying HPV types 16 and 18 from clinical samples.
Files in This Item:
T201202601.pdf Download
DOI
22518863
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Yong Jung(박용정)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90693
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