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Fabrication of cross-linked alginate beads using electrospraying for adenovirus delivery.

Authors
 Hongkwan Park ; Pyung-Hwan Kim ; Jung Hyun Kim ; Chae-Ok Yun ; Sung-Wook Choi ; Tae-Joon Park ; Oh-Joon Kwon ; Taewon Hwang 
Citation
 International Journal of Pharmaceutics, Vol.427(2) : 417~425, 2012 
Journal Title
 International Journal of Pharmaceutics 
ISSN
 0378-5173 
Issue Date
2012
Abstract
Cross-linked alginate beads containing adenovirus (Ad) were successfully fabricated using an electrospraying method to achieve the protection and release of Ad in a controlled manner. An aqueous alginate solution containing Ad was electrosprayed into an aqueous phase containing a cross-linking agent (calcium chloride) at different process variables (voltages, alginate concentrations, and flow rates). Alginate beads containing Ad were used for transduction of U343 glioma cells and the transduction efficiency of the alginate beads was measured by quantification of gene expression using a fluorescence-activated cell sorter at different time points. In vitro results of gene expression revealed that the Ad encapsulated in the alginate beads with 0.5 wt% of alginate concentration exhibited a high activity for a long period (over 7 days) and was released in a sustained manner from the alginate beads. The Ad-encapsulating alginate beads could be promising materials for local delivery of Ad at a high concentration into target sites.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/90516
DOI
10.1016/j.ijpharm.2012.01.050
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Institute for Cancer Research
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0378517312001044
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