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Fabrication of cross-linked alginate beads using electrospraying for adenovirus delivery.

Authors
 Hongkwan Park  ;  Pyung-Hwan Kim  ;  Taewon Hwang  ;  Oh-Joon Kwon  ;  Tae-Joon Park  ;  Sung-Wook Choi  ;  Chae-Ok Yun  ;  Jung Hyun Kim 
Citation
 INTERNATIONAL JOURNAL OF PHARMACEUTICS, Vol.427(2) : 417-425, 2012 
Journal Title
 INTERNATIONAL JOURNAL OF PHARMACEUTICS 
ISSN
 0378-5173 
Issue Date
2012
MeSH
Adenoviridae/genetics* ; Alginates/chemistry* ; Calcium Chloride ; Cell Line, Tumor ; Cross-Linking Reagents/chemistry* ; Delayed-Action Preparations ; Drug Compounding ; Electrochemistry ; Excipients ; Flow Cytometry ; Genetic Therapy ; Genetic Vectors/administration & dosage* ; Green Fluorescent Proteins/chemistry ; Green Fluorescent Proteins/genetics ; Humans ; Neoplasms/therapy ; Particle Size ; Solutions ; Transduction, Genetic
Keywords
Electrospraying ; Encapsulation ; Adenovirus ; Alginate ; Controlled release
Abstract
Cross-linked alginate beads containing adenovirus (Ad) were successfully fabricated using an electrospraying method to achieve the protection and release of Ad in a controlled manner. An aqueous alginate solution containing Ad was electrosprayed into an aqueous phase containing a cross-linking agent (calcium chloride) at different process variables (voltages, alginate concentrations, and flow rates). Alginate beads containing Ad were used for transduction of U343 glioma cells and the transduction efficiency of the alginate beads was measured by quantification of gene expression using a fluorescence-activated cell sorter at different time points. In vitro results of gene expression revealed that the Ad encapsulated in the alginate beads with 0.5 wt% of alginate concentration exhibited a high activity for a long period (over 7 days) and was released in a sustained manner from the alginate beads. The Ad-encapsulating alginate beads could be promising materials for local delivery of Ad at a high concentration into target sites.
Full Text
http://www.sciencedirect.com/science/article/pii/S0378517312001044
DOI
22326298
Appears in Collections:
5. Research Institutes (연구소) > Institute for Cancer Research (암연구소) > 1. Journal Papers
Yonsei Authors
Yun, Chae Ok(윤채옥)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90516
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